Humboldt-Universität zu Berlin - Structural Biology / Biochemistry

2025

Basak Yudhajeet, Lorent Christian, Jeoung Jae-Hun, Zebger Ingo, Dobbek Holger Metalloradical-driven enzymatic CO2 reduction by a dynamic Ni–Fe cluster Nature Catalysis. 10.1038/s41929-025-01388-5

Ruickoldt Jakob, Kreibich Julian, Bick Thomas, Jeoung Jae-Hun, Duffus Benjamin R., Leimkühler Silke, Dobbek Holger, Wendler Petra Ligand binding to a Ni–Fe cluster orchestrates conformational changes of the CO-dehydrogenase–acetyl-CoA synthase complex Nature Catalysis. 657-667. 10.1038/s41929-025-01365-y

2024

Ruickoldt Jakob, Jeoung Jae‐Hun, Rudolph Maik Alexander, Lennartz Frank, Kreibich Julian, Schomäcker Reinhard, Dobbek Holger Coupling CO2 Reduction and Acetyl‐CoA Formation: The Role of a CO Capturing Tunnel in Enzymatic Catalysis Angewandte Chemie International Edition. 10.1002/anie.202405120

Hussein Rana, Graça André, Forsman Jack, Aydin A. Orkun, Hall Michael, Gaetcke Julia, Chernev Petko, Wendler Petra, Dobbek Holger, Messinger Johannes, Zouni Athina, Schröder Wolfgang P. Cryo–electron microscopy reveals hydrogen positions and water networks in photosystem II Science. 1349-1355. 10.1126/science.adn6541

Barthel Tatjana, Benz Laila, Basler Yara, Crosskey Thomas, Dillmann Alexander, Förster Ronald, Fröling Paula, Dieguez Camilla G., Gless Christine, Hauß Thomas, Hellmig Michael, Jänisch Lea, James David, Lennartz Frank, Mijatovic Jelena, Oelker Melanie, Scanlan James W., Weber Gert, Wollenhaupt Jan, Mueller Uwe, Dobbek Holger, Wahl Markus C., Weiss Manfred S. The HZB F2X‐Facility—An Efficient Crystallographic Fragment Screening Platform Applied Research. 10.1002/appl.202400110

Jeoung Jae‐Hun, Dobbek Holger Double Cubane Cluster Protein and Its Reductase 1-14. 10.1002/9781119951438.eibc2870

Panwar Alka, Martins Berta M., Sommer Frederik, Schroda Michael, Dobbek Holger, Iobbi-Nivol Chantal, Jourlin-Castelli Cécile, Leimkühler Silke Purification and Electron Transfer from Soluble c-Type Cytochrome TorC to TorA for Trimethylamine N-Oxide Reduction International Journal of Molecular Sciences. 13331. 10.3390/ijms252413331

Žoldák Gabriel, Knappe Thomas A., Geitner Anne-Juliane, Scholz Christian, Dobbek Holger, Schmid Franz X., Jakob Roman P. Bacterial Chaperone Domain Insertions Convert Human FKBP12 into an Excellent Protein-Folding Catalyst—A Structural and Functional Analysis Molecules. 1440. 10.3390/molecules29071440

2023

Bhowmick Asmit, Simon Philipp S., Bogacz Isabel, Hussein Rana, Zhang Miao, Makita Hiroki, Ibrahim Mohamed, Chatterjee Ruchira, Doyle Margaret D., Cheah Mun Hon, Chernev Petko, Fuller Franklin D., Fransson Thomas, Alonso-Mori Roberto, Brewster Aaron S., Sauter Nicholas K., Bergmann Uwe, Dobbek Holger, Zouni Athina, Messinger Johannes, Kern Jan, Yachandra Vittal K., Yano Junko Going around the Kok cycle of the water oxidation reaction with femtosecond X-ray crystallography IUCrJ. 642-655. 10.1107/S2052252523008928

Sigurdsson Arnar, Martins Berta M., Düttmann Simon A., Jasyk Martin, Dimos‐Röhl Benjamin, Schöpf Felix, Gemander Manuel, Knittel Caroline H., Schnegotzki Romina, Schmid Bianca, Kosol Simone, Pommerening Lea, Gonzáles‐Viegaz María, Seidel Maria, Hügelland Manuela, Leimkühler Silke, Dobbek Holger, Mainz Andi, Süssmuth Roderich D. Discovery of the Lanthipeptide Curvocidin and Structural Insights into its Trifunctional Synthetase CuvL Angewandte Chemie International Edition. 10.1002/anie.202302490

Bhowmick Asmit, Hussein Rana, Bogacz Isabel, Simon Philipp S., Ibrahim Mohamed, Chatterjee Ruchira, Doyle Margaret D., Cheah Mun Hon, Fransson Thomas, Chernev Petko, Kim In-Sik, Makita Hiroki, Dasgupta Medhanjali, Kaminsky Corey J., Zhang Miao, Gätcke Julia, Haupt Stephanie, Nangca Isabela I., Keable Stephen M., Aydin A. Orkun, Tono Kensuke, Owada Shigeki, Gee Leland B., Fuller Franklin D., Batyuk Alexander, Alonso-Mori Roberto, Holton James M., Paley Daniel W., Moriarty Nigel W., Mamedov Fikret, Adams Paul D., Brewster Aaron S., Dobbek Holger, Sauter Nicholas K., Bergmann Uwe, Zouni Athina, Messinger Johannes, Kern Jan, Yano Junko, Yachandra Vittal K. Structural evidence for intermediates during O2 formation in photosystem II Nature. 629-636. 10.1038/s41586-023-06038-z

Basak Yudhajeet, Jeoung Jae‐Hun, Domnik Lilith, Dobbek Holger Stepwise O2‐Induced Rearrangement and Disassembly of the [NiFe4(OH)(μ3‐S)4] Active Site Cluster of CO Dehydrogenase Angewandte Chemie International Edition. 10.1002/anie.202305341

Hussein Rana, Ibrahim Mohamed, Bhowmick Asmit, Simon Philipp S., Bogacz Isabel, Doyle Margaret D., Dobbek Holger, Zouni Athina, Messinger Johannes, Yachandra Vittal K., Kern Jan F., Yano Junko Evolutionary diversity of proton and water channels on the oxidizing side of photosystem II and their relevance to function Photosynthesis Research. 91-107. 10.1007/s11120-023-01018-w

2022

Ruickoldt Jakob, Basak Yudhajeet, Domnik Lilith, Jeoung Jae-Hun, Dobbek Holger On the Kinetics of CO2 Reduction by Ni, Fe-CO Dehydrogenases ACS Catalysis. 13131-13142. 10.1021/acscatal.2c02221

Jeoung J.H., Nicklisch S., Dobbek H. Structural basis for coupled ATP-driven electron transfer in the double-cubane cluster protein Proceedings of the National Academy of Sciences of the United States of America. 31. 10.1073/pnas.2203576119

Basak Yudhajeet, Jeoung Jae-Hun, Domnik Lilith, Ruickoldt Jakob, Dobbek Holger Substrate Activation at the Ni,Fe Cluster of CO Dehydrogenases: The Influence of the Protein Matrix ACS Catalysis. 12711-12719. 10.1021/acscatal.2c02922

Lennartz F., Jeoung J.H., Ruenger S., Dobbek H., Weiss M.S. Determining the oxidation state of elements by X-ray crystallography Acta Crystallographica Section D: Structural Biology. 238-247. 10.1107/S2059798321013048

Jeoung J.H., Fesseler J., Domnik L., Klemke F., Sinnreich M., Teutloff C., Dobbek H. A Morphing [4Fe-3S-nO]-Cluster within a Carbon Monoxide Dehydrogenase Scaffold Angewandte Chemie - International Edition. 18. 10.1002/anie.202117000

Simon Philipp S., Makita Hiroki, Bogacz Isabel, Fuller Franklin, Bhowmick Asmit, Hussein Rana, Ibrahim Mohamed, Zhang Miao, Chatterjee Ruchira, Cheah Mun Hon, Chernev Petko, Doyle Margaret D., Brewster Aaron S., Alonso‐Mori Roberto, Sauter Nicholas K., Bergmann Uwe, Dobbek Holger, Zouni Athina, Messinger Johannes, Kern Jan, Yachandra Vittal K., Yano Junko Capturing the sequence of events during the water oxidation reaction in photosynthesis using XFELs FEBS Letters. 30-37. 10.1002/1873-3468.14527

2021

Ibrahim M., Moriarty N.W., Kern J., Holton J.M., Brewster A.S., Bhowmick A., Bergmann U., Zouni A., Messinger J., Yachandra V.K., Yano J., Dobbek H., Sauter N.K., Adams P.D. Clear evidence of binding of Ox to the oxygen-evolving complex of photosystem II is best observed in the omit map Proceedings of the National Academy of Sciences of the United States of America. 24. 10.1073/pnas.2102342118

Hussein R., Ibrahim M., Bhowmick A., Simon P.S., Chatterjee R., Lassalle L., Doyle M., Bogacz I., Kim I.S., Cheah M.H., Gul S., de Lichtenberg C., Chernev P., Pham C.C., Young I.D., Carbajo S., Fuller F.D., Alonso-Mori R., Batyuk A., Sutherlin K.D., Brewster A.S., Bolotovsky R., Mendez D., Holton J.M., Moriarty N.W., Adams P.D., Bergmann U., Sauter N.K., Dobbek H., Messinger J., Zouni A., Kern J., Yachandra V.K., Yano J. Structural dynamics in the water and proton channels of photosystem II during the S2 to S3 transition Nature Communications. 1. 10.1038/s41467-021-26781-z

Jeoung J.H., Rünger S., Haumann M., Neumann B., Klemke F., Davis V., Fischer A., Dau H., Wollenberger U., Dobbek H. Bimetallic Mn, Fe, Co, and Ni Sites in a Four-Helix Bundle Protein: Metal Binding, Structure, and Peroxide Activation Inorganic Chemistry. 23. 17498-17508. 10.1021/acs.inorgchem.1c01919

Neumann F., Dobbek H. ATP Binding and a Second Reduction Enables a Conformationally Gated Uphill Electron Transfer ACS Catalysis. 14. 8565-8575. 10.1021/acscatal.1c01038

2020

Jeoung J.H., Martins B.M., Dobbek H. Double-Cubane [8Fe9S] Clusters: A Novel Nitrogenase-Related Cofactor in Biology ChemBioChem. 12. 1710-1716. 10.1002/cbic.202000016

Gerland L., Friedrich D., Hopf L., Donovan E.J., Wallmann A., Erdmann N., Diehl A., Bommer M., Buzar K., Ibrahim M., Schmieder P., Dobbek H., Zouni A., Bondar A.N., Dau H., Oschkinat H. pH-Dependent Protonation of Surface Carboxylate Groups in PsbO Enables Local Buffering and Triggers Structural Changes ChemBioChem. 11. 1597-1604. 10.1002/cbic.201900739

Ibrahim M., Fransson T., Chatterjee R., Cheah M.H., Hussein R., Lassalle L., Sutherlin K.D., Young I.D., Fuller F.D., Gul S., Kim I.S., Simon P.S., de Lichtenberg C., Chernev P., Bogacz I., Pham C.C., Orville A.M., Saichek N., Northen T., Batyuk A., Carbajo S., Alonso-Mori R., Tono K., Owada S., Bhowmick A., Bolotovsky R., Mendez D., Moriarty N.W., Holton J.M., Dobbek H., Brewster A.S., Adams P.D., Sauter N.K., Bergmann U., Zouni A., Messinger J., Kern J., Yachandra V.K., Yano J. Untangling the sequence of events during the S2 → S3 transition in photosystem II and implications for the water oxidation mechanism Proceedings of the National Academy of Sciences of the United States of America. 23. 12624-12635. 10.1073/pnas.2000529117

Benvenuti M., Meneghello M., Guendon C., Jacq-Bailly A., Jeoung J.H., Dobbek H., Léger C., Fourmond V., Dementin S. The two CO-dehydrogenases of Thermococcus sp. AM4 Biochimica et Biophysica Acta - Bioenergetics. 7. 10.1016/j.bbabio.2020.148188

Ilina Y., Martins B.M., Jeoung J.H., Dobbek H. Nickel, Iron, Sulfur Sites Metal ions in life sciences. 10.1515/9783110589757-017

2019

Han R., Rempfer K., Zhang M., Dobbek H., Zouni A., Dau H., Luber S. Investigating the Structure and Dynamics of Apo-Photosystem II ChemCatChem. 16. 4072-4080. 10.1002/cctc.201900351

Jeoung JH, Martins BM, Dobbek H X-Ray Crystallography of Carbon Monoxide Dehydrogenases Methods Mol Biol. 167-178. 10.1007/978-1-4939-8864-8_11

Ilina Y., Lorent C., Katz S., Jeoung J.H., Shima S., Horch M., Zebger I., Dobbek H. X-ray Crystallography and Vibrational Spectroscopy Reveal the Key Determinants of Biocatalytic Dihydrogen Cycling by [NiFe] Hydrogenases Angewandte Chemie - International Edition. 51. 18710-18714. 10.1002/anie.201908258

Baumann T, Hauf M, Schildhauer F, Eberl KB, Durkin PM, Deniz E, Lö}ffler JG, Acevedo-Rocha CG, Jaric J, Martins BM, Dobbek H, Bredenbeck J, Budisa N Site-Resolved Observation of Vibrational Energy Transfer Using a Genetically Encoded Ultrafast Heater Angew Chem Int Ed Engl. 9. 2899-2903. 10.1002/anie.201812995

Dobbek H. Structure and Mechanism of Ni- and Fe-containing Carbon Monoxide Dehydrogenases Oxygen Production and Reduction in Artificial and Natural Systems. 307-318. 10.1142/9789813276925_0015

Kemmler L., Ibrahim M., Dobbek H., Zouni A., Bondar A.N. Dynamic water bridging and proton transfer at a surface carboxylate cluster of photosystem II Physical Chemistry Chemical Physics. 45. 25449-25466. 10.1039/c9cp03926k

2018

Wilk P., Uehlein M., Piwowarczyk R., Dobbek H., Mueller U., Weiss M. Structural basis for prolidase deficiency disease mechanisms FEBS Journal. 18. 3422-3441. 10.1111/febs.14620

Keller S., Kunze C., Bommer M., Paetz C., Menezes R., Svatoš A., Dobbek H., Schubert T. Selective utilization of benzimidazolylnorcobamides as cofactors by the tetrachloroethene reductive dehalogenase of Sulfurospirillum multivorans Journal of Bacteriology. 8. 10.1128/JB.00584-17

Kern J., Chatterjee R., Young I.D., Fuller F.D., Lassalle L., Ibrahim M., Gul S., Fransson T., Brewster A.S., Alonso-Mori R., Hussein R., Zhang M., Douthit L., de Lichtenberg C., Cheah M.H., Shevela D., Wersig J., Seuffert I., Sokaras D., Pastor E., Weninger C., Kroll T., Sierra R.G., Aller P., Butryn A., Orville A.M., Liang M., Batyuk A., Koglin J.E., Carbajo S., Boutet S., Moriarty N.W., Holton J.M., Dobbek H., Adams P.D., Bergmann U., Sauter N.K., Zouni A., Messinger J., Yano J., Yachandra V.K. Structures of the intermediates of Kok’s photosynthetic water oxidation clock Nature. 7731. 421-425. 10.1038/s41586-018-0681-2

Sullivan B., Archibald R., Langan P.S., Dobbek H., Bommer M., McFeeters R.L., Coates L., Wang X., Gallmeier F., Carpenter J.M., Lynch V., Langan P. Improving the accuracy and resolution of neutron crystallographic data by three-dimensional profile fitting of Bragg peaks in reciprocal space Acta Crystallographica Section D: Structural Biology. 11. 1085-1095. 10.1107/S2059798318013347

Stehling O., Jeoung J.H., Freibert S.A., Paul V.D., Bänfer S., Niggemeyer B., Rösser R., Dobbek H., Lill R. Function and crystal structure of the dimeric P-loop ATPase CFD1 coordinating an exposed [4Fe-4S] cluster for transfer to apoproteins Proceedings of the National Academy of Sciences of the United States of America. 39. E9085-E9094. 10.1073/pnas.1807762115

Jeoung J., Dobbek H. ATP-dependent substrate reduction at an [Fe8S9] double-cubane cluster Proceedings of the National Academy of Sciences of the United States of America. 12. 2994-2999. 10.1073/pnas.1720489115

Hussein R., Ibrahim M., Chatterjee R., Coates L., Müh F., Yachandra V.K., Yano J., Kern J., Dobbek H., Zouni A. Optimizing Crystal Size of Photosystem II by Macroseeding: Toward Neutron Protein Crystallography Crystal Growth and Design. 1. 85-94. 10.1021/acs.cgd.7b00878

2017

Werther T., Wahlefeld S., Salewski J., Kuhlmann U., Zebger I., Hildebrandt P., Dobbek H. Redox-dependent substrate-cofactor interactions in the Michaelis-complex of a flavin-dependent oxidoreductase Nature Communications

Hauf M, Richter F, Schneider T, Faidt T, Martins BM, Baumann T, Durkin P, Dobbek H, Jacobs K, Moeglich A, Budisa N Photoactivatable Mussel-Based Underwater Adhesive Proteins by an Expanded Genetic Code ChemBioChem. 1-6. 10.1002/cbic.201700327

Ciaccafava A., Tombolelli D., Domnik L., Jeoung J.H., Dobbek H., Mroginski M.A., Zebger I., Hildebrandt P. Carbon Monoxide Dehydrogenase Reduces Cyanate to Cyanide Angewandte Chemie - International Edition. 26. 7398-7401. 10.1002/anie.201703225

Kunze C., Bommer M., Hagen W., Uksa M., Dobbek H., Schubert T., Diekert G. Cobamide-mediated enzymatic reductive dehalogenation via long-range electron transfer Nature Communications. 10.1038/ncomms15858

Domnik L., Merrouch M., Goetzl S., Jeoung J.H., Léger C., Dementin S., Fourmond V., Dobbek H. CODH-IV: A High-Efficiency CO-Scavenging CO Dehydrogenase with Resistance to O2 Angewandte Chemie - International Edition. 48. 15466-15469. 10.1002/anie.201709261

Kielb P., Utesch T., Kozuch J., Jeoung J., Dobbek H., Mroginski M., Hildebrandt P., Weidinger I. Switchable Redox Chemistry of the Hexameric Tyrosine-Coordinated Heme Protein Journal of Physical Chemistry B. 16. 3955-3964

Wilk P., Uehlein M., Kalms J., Dobbek H., Mueller U., Weiss M. Substrate specificity and reaction mechanism of human prolidase FEBS Journal. 17. 2870-2885

Homburg C., Bommer M., Wuttge S., Hobe C., Beck S., Dobbek H., Deutscher J., Licht A., Schneider E. Inducer exclusion in Firmicutes: insights into the regulation of a carbohydrate ATP binding cassette transporter from Lactobacillus casei BL23 by the signal transducing protein P-Ser46-HPr Molecular Microbiology. 1. 25-45. 10.1111/mmi.13680

Schrapers P., Ilina J., Gregg C.M., Mebs S., Jeoung J.H., Dau H., Dobbek H., Haumann M. Ligand binding at the A-cluster in full-length or truncated acetyl-CoA synthase studied by X-ray absorption spectroscopy PLoS ONE. 2. 10.1371/journal.pone.0171039

Dobbek H. Nitrogenase: A fuel-producing microbe Nature Chemical Biology. 2. 134-135. 10.1038/nchembio.2288

Zhang M., Bommer M., Chatterjee R., Hussein R., Yano J., Dau H., Kern J., Dobbek H., Zouni A. Structural insights into the light-driven auto-assembly process of the wateroxidizing Mn4CaO5-cluster in photosystem II eLife

Dobbek H. Playing marble run to make methane Science. 6352. 642-643. 10.1126/science.aao2439

Bommer M., Coates L., Dau H., Zouni A., Dobbek H. Protein crystallization and initial neutron diffraction studies of the photosystem II subunit PsbO Acta Crystallographica Section:F Structural Biology Communications. 9. 525-531

Goetzl S., Teutloff C., Werther T., Hennig S., Jeoung J., Bittl R., Dobbek H. Protein Dynamics in the Reductive Activation of a B12-Containing Enzyme Biochemistry. 41. 5496-5502

2016

Peng L., Yarman A., Jetzschmann K., Jeoung J., Schad D., Dobbek H., Wollenberger U., Scheller F. Molecularly imprinted electropolymer for a hexameric heme protein with direct electron transfer and peroxide electrocatalysis Sensors (Switzerland). 3

Bommer M., Bondar A., Zouni A., Dobbek H., Dau H. Crystallographic and Computational Analysis of the Barrel Part of the PsbO Protein of Photosystem II: Carboxylate-Water Clusters as Putative Proton Transfer Relays and Structural Switches Biochemistry. 33. 4626-4635

Dobbek H., Leys D. Insights into reductive dehalogenase function obtained from crystal structures Organohalide-Respiring Bacteria. 485-495

Schäfer C., Bommer M., Hennig S., Jeoung J., Dobbek H., Lenz O. Structure of an Actinobacterial-Type [NiFe]-Hydrogenase Reveals Insight into O2-Tolerant H2 Oxidation Structure. 2. 285-292

Jeoung J., Nianios D., Fetzner S., Dobbek H. Quercetin 2,4-Dioxygenase Activates Dioxygen in a Side-On O2-Ni Complex Angewandte Chemie - International Edition. 10. 3281-3284

Schrapers P., Mebs S., Ilina Y., Warner D., Wörmann C., Schuth N., Kositzki R., Dau H., Limberg C., Dobbek H., Haumann M. The binuclear nickel center in the A-cluster of acetyl-CoA synthase (ACS) and two biomimetic dinickel complexes studied by X-ray absorption and emission spectroscopy Journal of Physics: Conference Series

Schrapers P., Mebs S., Goetzl S., Hennig S., Dau H., Dobbek H., Haumann M. Axial ligation and redox changes at the cobalt ion in cobalamin bound to Corrinoid Iron-Sulfur Protein (CoFeSP) or in solution characterized by XAS and DFT PLoS ONE. 7

Gregg C.M., Goetzl S., Jeoung J.H., Dobbek H. AcsF catalyzes the ATP-dependent insertion of Nickel into the Ni, Ni-[4Fe4S] cluster of Acetyl-CoA synthase Journal of Biological Chemistry. 35. 18129-18138

Ciaccafava A., Tombolelli D., Domnik L., Fesseler J., Jeoung J., Dobbek H., Mroginski M., Zebger I., Hildebrandt P. When the inhibitor tells more than the substrate: The cyanide-bound state of a carbon monoxide dehydrogenase Chemical Science. 5. 3162-3171

Young I.D., Ibrahim M., Chatterjee R., Gul S., Fuller F.D., Koroidov S., Brewster A.S., Tran R., Alonso-Mori R., Kroll T., Michels-Clark T., Laksmono H., Sierra R.G., Stan C.A., Hussein R., Zhang M., Douthit L., Kubin M., De Lichtenberg C., Vo Pham L., Nilsson H., Cheah M.H., Shevela D., Saracini C., Bean M.A., Seuffert I., Sokaras D., Weng T.C., Pastor E., Weninger C., Fransson T., Lassalle L., Bräuer P., Aller P., Docker P.T., Andi B., Orville A.M., Glownia J.M., Nelson S., Sikorski M., Zhu D., Hunter M.S., Lane T.J., Aquila A., Koglin J.E., Robinson J., Liang M., Boutet S., Lyubimov A.Y., Uervirojnangkoorn M., Moriarty N.W., Liebschner D., Afonine P.V., Waterman D.G., Evans G., Wernet P., Dobbek H., Weis W.I., Brunger A.T., Zwart P.H., Adams P.D., Zouni A., Messinger J., Bergmann U., Sauter N.K., Kern J., Yachandra V.K., Yano J. Structure of photosystem II and substrate binding at room temperature Nature. 7633. 453-457

2015

Tanne J., Jeoung J.H., Peng L., Yarman A., Dietzel B., Schulz B., Schad D., Dobbek H., Wollenberger U., Bier F.F., Scheller F.W. Direct Electron Transfer and Bioelectrocatalysis by a Hexameric, Heme Protein at Nanostructured Electrodes Electroanalysis. 10. 2262-2267

Peng L., Utesch T., Yarman A., Jeoung J., Steinborn S., Dobbek H., Mroginski M., Tanne J., Wollenberger U., Scheller F. Surface-tuned electron transfer and electrocatalysis of hexameric tyrosine-coordinated heme protein Chemistry - A European Journal. 20. 7596-7602

Fesseler J., Jeoung J., Dobbek H. How the [NiFe4S4] Cluster of CO Dehydrogenase Activates CO2 and NCO- Angewandte Chemie - International Edition. 29. 8560-8564

Merrouch M., Hadj-Saïd J., Domnik L., Dobbek H., Léger C., Dementin S., Fourmond V. O2 Inhibition of Ni-Containing CO Dehydrogenase Is Partly Reversible Chemistry - A European Journal. 52. 18934-18938

2014

Hennig S., Goetzl S., Jeoung J., Bommer M., Lendzian F., Hildebrandt P., Dobbek H. ATP-induced electron transfer by redox-selective partner recognition Nature Communications

Jeoung J.H., Fesseler J., Goetzl S., Dobbek H. Carbon monoxide. Toxic gas and fuel for anaerobes and aerobes: Carbon monoxide dehydrogenases Metal Ions in Life Sciences. 37-69

Hellmich J., Bommer M., Burkhardt A., Ibrahim M., Kern J., Meents A., Müh F., Dobbek H., Zouni A. Native-like Photosystem II Superstructure at 2.44 Å Resolution through Detergent Extraction from the Protein Crystal Structure. 11. 1607-1615

Bommer M., Kunze C., Fesseler J., Schubert T., Diekert G., Dobbek H. Structural basis for organohalide respiration Science. 6208. 455-458

Jeoung J., Goetzl S., Hennig S., Fesseler J., Wörmann C., Dendra J., Dobbek H. The extended reductive acetyl-CoA pathway:ATpases in metal cluster maturation and reductive activation Biological Chemistry. 5. 545-558

2013

Nehrkorn J, Martins BM, Holldack K, Stoll S, Dobbek H, Bittl R, Schnegg A Zero-field splittings in metHb and metMb with aquo and fluoro ligands: a FD-FT THz-EPR study Mol Phys. 2696-2707. doi:10.1080/00268976.2013.809806

Jeoung J., Bommer M., Lin T., Dobbek H. Visualizing the substrate-, superoxo-, alkylperoxo-, and product-bound states at the nonheme Fe(II) site of homogentisate dioxygenase Proceedings of the National Academy of Sciences of the United States of America. 31. 12625-12630

Appel A., Bercaw J., Bocarsly A., Dobbek H., Dubois D., Dupuis M., Ferry J., Fujita E., Hille R., Kenis P., Kerfeld C., Morris R., Peden C., Portis A., Ragsdale S., Rauchfuss T., Reek J., Seefeldt L., Thauer R., Waldrop G. Frontiers, opportunities, and challenges in biochemical and chemical catalysis of CO2 fixation Chemical Reviews. 8. 6621-6658

2012

Buckel W, Zhang J, Friedrich P, Parthasarathy A, Li H, Djurdjevic I, Dobbek H, Martins BM Enzyme catalyzed radical dehydrations of hydroxy acids Biochim Biophys Acta. 1278-1290

Hennig S., Jeoung J., Goetzl S., Dobbek H. Redox-dependent complex formation by an ATP-dependent activator of the corrinoid/iron-sulfur protein Proceedings of the National Academy of Sciences of the United States of America. 14. 5235-5240

Knauer S., Buckel W., Dobbek H. On the ATP-dependent activation of the radical enzyme (R)-2- hydroxyisocaproyl-CoA dehydratase Biochemistry. 33. 6609-6622

Jeoung J., Dobbek H. n-Butyl isocyanide oxidation at the [NiFe 4S 4OH x] cluster of CO dehydrogenase Journal of Biological Inorganic Chemistry. 2. 167-173

Jakob R., Geitner A., Weininger U., Balbach J., Dobbek H., Schmid F. Structural and energetic basis of infection by the filamentous bacteriophage IKe Molecular Microbiology. 6. 1124-1138

Meister W., Hennig S., Jeoung J., Lendzian F., Dobbek H., Hildebrandt P. Complex formation with the activator RACo affects the corrinoid structure of CoFeSP Biochemistry. 36. 7040-7042

Lin T.Y., Werther T., Jeoung J.H., Dobbek H. Suppression of electron transfer to dioxygen by charge transfer and electron transfer complexes in the FAD-dependent reductase componentof toluene dioxygenase Journal of Biological Chemistry. 45. 38338-38346

Wuttge S., Bommer M., Jäger F., Martins B., Jacob S., Licht A., Scheffel F., Dobbek H., Schneider E. Determinants of substrate specificity and biochemical properties of the sn-glycerol-3-phosphate ATP binding cassette transporter (UgpB-AEC2) of Escherichia coli Molecular Microbiology. 4. 908-920

Knauer S.H., Hartl-Spiegelhauer O., Schwarzinger S., Hänzelmann P., Dobbek H. The Fe(II)/ α -ketoglutarate-dependent taurine dioxygenases from Pseudomonas putida and Escherichia coli are tetramers FEBS Journal. 5. 816-831

2011

Knauer S., Buckel W., Dobbek H. Structural basis for reductive radical formation and electron recycling in (R)-2-hydroxyisocaproyl-CoA dehydratase Journal of the American Chemical Society. 12. 4342-4347

Goetzl S., Jeoung J.H., Hennig S.E., Dobbek H. Structural basis for electron and methyl-group transfer in a methyltransferase system operating in the reductive acetyl-CoA pathway Journal of Molecular Biology. 1. 96-109

Dobbek H. Structural aspects of mononuclear Mo/W-enzymes Coordination Chemistry Reviews. 1104-1116

Lorenz S.H., Jakob R.P., Weininger U., Balbach J., Dobbek H., Schmid F.X. The filamentous phages fd and IF1 use different mechanisms to infect escherichia coli Journal of Molecular Biology. 4. 989-1003

2010

Spiegelhauer O., Mende S., Dickert F., Knauer S.H., Ullmann G.M., Dobbek H. Cysteine as a modulator residue in the active site of xenobiotic reductase A: A structural, thermodynamic and kinetic study Journal of Molecular Biology. 1. 66-82

Jeoung J.H., Giese T., Grünwald M., Dobbek H. Crystal structure of the ATP-dependent maturation factor of Ni,Fe-containing carbon monoxide dehydrogenases Journal of Molecular Biology. 4. 1165-1179

Jakob R.P., Zierer B.K., Weininger U., Hofmann S.D., Lorenz S.H., Balbach J., Dobbek H., Schmid F.X. Elimination of a Cis-proline-containing loop and turn optimization stabilizes a protein and accelerates its folding Journal of Molecular Biology. 2. 331-346

Spiegelhauer O., Werther T., Mende S., Knauer S.H., Dobbek H. Determinants of Substrate Binding and Protonation in the Flavoenzyme Xenobiotic Reductase A Journal of Molecular Biology. 2. 286-298

2009

Jeoung J., Dobbek H. Structural basis of cyanide inhibition of Ni, Fe-containing carbon monoxide dehydrogenase Journal of the American Chemical Society. 29. 9922-9923

Wagener N., Pierik A., Ibdah A., Hille R., Dobbek H. The Mo-Se active site of nicotinate dehydrogenase Proceedings of the National Academy of Sciences of the United States of America. 27. 11055-11060

Spiegelhauer O., Dickert F., Mende S., Niks D., Hille R., Ullmann M., Dobbek H. Kinetic characterization of xenobiotic reductase A from Pseudomonas putida 86 Biochemistry. 48. 11412-11420

Jeoung J.H., Giese T., Grünwald M., Dobbek H. CooC1 from Carboxydothermus hydrogenoformans is a nickel-binding ATPase Biochemistry. 48. 11505-11513

2008

Kather I., Jakob R., Dobbek H., Schmid F.X. Changing the Determinants of Protein Stability from Covalent to Non-Covalent Interactions by In Vitro Evolution: A Structural and Energetic Analysis Journal of Molecular Biology. 4. 1040-1054

Kather I., Jakob R.P., Dobbek H., Schmid F.X. Increased Folding Stability of TEM-1 β-Lactamase by In Vitro Selection Journal of Molecular Biology. 1. 238-251

Dobbek H. How biological Fe-S centers activate CO2 Wie Biologische Fe-S-Zentren CO2 Aktivieren Nachrichten aus der Chemie. 734-737

2007

Jeoung J, Pippig DA, Martins BM, Wagener N, Dobbek H HTHP: a novel class of hexameric, tyrosine-coordinated heme proteins Journal of Molecular Biology. 4. 1122{--}1131

Einsle O., Andrade S., Dobbek H., Meyer J., Rees D. Assignment of individual metal redox states in a metalloprotein by crystallographic refinement at multiple X-ray wavelengths Journal of the American Chemical Society. 8. 2210-2211

Zoldák G., Redecke L., Svergun D.I., Konarev P.V., Voertler C.S., Dobbek H., Sedlák E., Sprinzl M. Release factors 2 from Escherichia coli and Thermus thermophilus: Structural, spectroscopic and microcalorimetric studies Nucleic Acids Research. 4. 1343-1353

Jeoung J.H., Dobbek H. Carbon dioxide activation at the Ni,Fe-cluster of anaerobic carbon monoxide dehydrogenase Science. 5855. 1461-1464

2006

Griese J.J., P. Jakob R., Schwarzinger S., Dobbek H. Xenobiotic Reductase A in the Degradation of Quinoline by Pseudomonas putida 86: Physiological Function, Structure and Mechanism of 8-Hydroxycoumarin Reduction Journal of Molecular Biology. 1. 140-152

Svetlitchnaia T., Svetlitchnyi V., Meyer O., Dobbek H. Structural insights into methyltransfer reactions of a corrinoid iron-sulfur protein involved in acetyl-CoA synthesis Proceedings of the National Academy of Sciences of the United States of America. 39. 14331-14336

2005

Resch M., Dobbek H., Meyer O. Structural and functional reconstruction in situ of the [CuSMoO 2] active site of carbon monoxide dehydrogenase from the carbon monoxide oxidizing eubacterium Oligotropha carboxidovorans Journal of Biological Inorganic Chemistry. 5. 518-528

Martins B., Svetlitchnaia T., Dobbek H. 2-Oxoquinoline 8-monooxygenase oxygenase component: Active site modulation by Rieske-[2Fe-2S] center oxidation/reduction Structure. 5. 817-824

2004

Dobbek H., Svetlitchnyi V., Liss J., Meyer O. Carbon Monoxide Induced Decomposition of the Active Site [Ni-4Fe-5S] Cluster of CO Dehydrogenase Journal of the American Chemical Society. 17. 5382-5387

Bonin I, Martins BM, Puranov V, Fetzner S, Huber R, Dobbek H Active site geometry and substrate recognition of the molybdenum hydroxylase quinoline 2-oxidoreductase Structure. 8. 1425{--}1435

Martins B., Dobbek H., Çinkaya I., Buckel W., Messerschmidt A. Crystal structure of 4-hydroxybutyryl-CoA dehydratase: Radical catalysis involving a [4Fe-4S] cluster and flavin Proceedings of the National Academy of Sciences of the United States of America. 44. 15645-15649

Svetlitchnyi V., Dobbek H., Meyer-Klaucke W., Meins T., Thiele B., Römer P., Huber R., Meyer O. A functional Ni-Ni-[4Fe-4S] cluster in the monomeric acetyl-CoA synthase from Carboxydothermus hydrogenoformans Proceedings of the National Academy of Sciences of the United States of America. 2. 446-451

2002

Dobbek H., Gremer L., Kiefersauer R., Huber R., Meyer O. Catalysis at a dinuclear [CuSMo(=O)OH] cluster in a CO dehydrogenase resolved at 1.1-Å resolution Proceedings of the National Academy of Sciences of the United States of America. 25. 15971-15976

Dobbek H., Huber R. The molybdenum and tungsten cofactors: A crystallographic view Metals Ions in Biological System: Molybdenum and Tungsten: Their Roles in Biological Processes. 227-263

2001

Dobbek H., Svetlitchnyi V., Gremer L., Huber R., Meyer O. Crystal structure of a carbon monoxide dehydrogenase reveals a [Ni-4Fe-5S] cluster Science. 5533. 1281-1285

2000

Hänzelmann P., Dobbek H., Gremer L., Huber R., Meyer O. The effect of intracellular molybdenum in Hydrogenophaga pseudoflava on the crystallographic structure of the seleno-molybdo-iron-sulfur flavoenzyme carbon monoxide dehydrogenase Journal of Molecular Biology. 5. 1221-1235

Kiefersauer R., Than M.E., Dobbek H., Gremer L., Melero M., Strobl S., Dias J.M., Soulimane T., Huber R. A novel free-mounting system for protein crystals: Transformation and improvement of diffraction power by accurately controlled humidity changes Journal of Applied Crystallography. 5. 1223-1230

Meyer O., Gremer L., Ferner R., Ferner M., Dobbek H., Gnida M., Meyer-Klaucke W., Huber R. The role of Se, Mo and Fe in the structure and function of carbon monoxide dehydrogenase Biological Chemistry. 865-876

Gremer L., Kellner S., Dobbek H., Huber R., Meyer O. Binding of flavin adenine dinucleotide to molybdenum-containing carbon monoxide dehydrogenase from Oligotropha carboxidovorans. Structural and functional analysis of a carbon monoxide dehydrogenase species in which the native flavoprotein has been replaced by its recombinant counterpart produced in Escherichia coli Journal of Biological Chemistry. 3. 1864-1872

1999

Dobbek H., Gremer L., Meyer O., Huber R. Crystal structure and mechanism of CO dehydrogenase, a molybdo iron-sulfur flavoprotein containing S-selanylcysteine Proceedings of the National Academy of Sciences of the United States of America. 16. 8884-8889