LOCUS       pAPHVII_pPH360      4967 bp ds-DNA     circular SYN 01-JUN-2016
DEFINITION  Marker plasmid for C. reinhardtii transformation with hygromycin selection. Design by Irina Sizova. Deposited by the Hegemann lab (contact: Simon Kelterborn; sk@chlamy.de; www.chlamy.de)
ACCESSION   .
VERSION     .
KEYWORDS    pAPHVII C.reinhardtii
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Montag, 29. Mai 2017 from SnapGene 2.8.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     reporter        1..159
                     /label="lacZ-a"
     primer_bind     141..157
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     primer_bind     167..185
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     promoter        222..496
                     /label="HSP70A"
     misc_feature    309
                     /label="C->G"
     promoter        515..730
                     /label="RBCS2"
     CDS             join(731..738,884..980,1125..2054)
                     /translation="EMTQESLLLLDRIDSDDSYASLRNDQEFWEVSRRASPADQAACL
                     QI*LATPALCRRRLLAPLSLSQAASNPASPFPFAAPGPPRPGGAGPARAPRAARARRE
                     HQPGAGGRARPRDQAVRRALVRPREPGQRERGLRRAGRRPRAGAPPAGPRRAAPRHRR
                     LALAVPGHEPHDRHHLAQRHGRHHRPQRPAGAGPRAGPRAGPPAPRAPDRQHRADPPQ
                     RGVPRAAARAPRRHRGGPPRLGLPVPAPAGPPGGLAARRGHPAGRPRAPLRARRPARH
                     QHLRGPGRDRGDRHRGLHRRVRRRQPLQPGAAAPERLPRRPRDPGGPAGRRPVEAHRG
                     LRPRAAGLHLPARLRGVRGDPPGPERLHRPRGAGPVPVGPPRHRPRRLITYGV*RI"
                     /label="APHVII(v2)"
     intron          739..883
                     /label="Intron RBCS2"
     intron          981..1125
                     /label="Intron RBCS2"
     terminator      2069..2301
                     /label="RBCS2"
     primer_bind     complement(2319..2338)
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /label="T3 promoter"
     primer_bind     complement(2359..2375)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    2383..2399
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(2407..2437)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2452..2473
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the
                     presence of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(2746..3365)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="pBR322 ori"
     CDS             complement(3520..4380)
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR (bla)"
     promoter        complement(4381..4485)
                     /label="AmpR promoter"
     rep_origin      4512..4967
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN
        1 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT
       61 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG
      121 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAG CGCGCGTAAT ACGACTCACT
      181 ATAGGGCGAA TTGGAGCTCC ACCGCGGTGG CGGCCGCTCT AGACGGCGGG GAGCTCGCTG
      241 AGGCTTGACA TGATTGGTGC GTATGTTTGT ATGAAGCTAC AGGACTGATT TGGCGGGCTA
      301 TGAGGGCGgG GGAAGCTCTG GAAGGGCCGC GATGGGGCGC GCGGCGTCCA GAAGGCGCCA
      361 TACGGCCCGC TGGCGGCACC CATCCGGTAT AAAAGCCCGC GACCCCGAAC GGTGACCTCC
      421 ACTTTCAGCG ACAAACGAGC ACTTATACAT ACGCGACTAT TCTGCCGCTA TACATAACCA
      481 CTCAGCTAGC TTAAGATCCC ATCAAGCTTG CATGCCGGGC GCGCCAGAAG GAGCGCAGCC
      541 AAACCAGGAT GATGTTTGAT GGGGTATTTG AGCACTTGCA ACCCTTATCC GGAAGCCCCC
      601 TGGCCCACAA AGGCTAGGCG CCAATGCAAG CAGTTCGCAT GCAGCCCCTG GAGCGGTGCC
      661 CTCCTGATAA ACCGGCCAGG GGGCCTATGT TCTTTACTTT TTTACAAGAG AAGTCACTCA
      721 ACATCTTAAA ATGGCCAGGT GAGTCGACGA GCAAGCCCGG CGGATCAGGC AGCGTGCTTG
      781 CAGATTTGAC TTGCAACGCC CGCATTGTGT CGACGAAGGC TTTTGGCTCC TCTGTCGCTG
      841 TCTCAAGCAG CATCTAACCC TGCGTCGCCG TTTCCATTTG CAGGATGCTC GAGATGACCC
      901 AGGAGAGCCT GCTGCTGCTG GACCGCATCG ACAGCGACGA CAGCTACGCC AGCCTGCGCA
      961 ACGACCAGGA GTTCTGGGAG GTGAGTCGAC GAGCAAGCCC GGCGGATCAG GCAGCGTGCT
     1021 TGCAGATTTG ACTTGCAACG CCCGCATTGT GTCGACGAAG GCTTTTGGCT CCTCTGTCGC
     1081 TGTCTCAAGC AGCATCTAAC CCTGCGTCGC CGTTTCCATT TGCAGCCCCT GGCCCGCCGC
     1141 GCCCTGGAGG AGCTGGGCCT GCCCGTGCCC CCCGTGCTGC GCGTGCCCGG CGAGAGCACC
     1201 AACCCGGTGC TGGTGGGCGA GCCCGGCCCC GTGATCAAGC TGTTCGGCGA GCACTGGTGC
     1261 GGCCCCGAGA GCCTGGCCAG CGAGAGCGAG GCCTACGCCG TGCTGGCCGA CGCCCCCGTG
     1321 CCGGTGCCCC GCCTGCTGGG CCGCGGCGAG CTGCGCCCCG GCACCGGCGC CTGGCCCTGG
     1381 CCGTACCTGG TCATGAGCCG CATGACCGGC ACCACCTGGC GCAGCGCCAT GGACGGCACC
     1441 ACCGACCGCA ACGCCCTGCT GGCGCTGGCC CGCGAGCTGG GCCGCGTGCT GGGCCGCCTG
     1501 CACCGCGTGC CCCTGACCGG CAACACCGTG CTGACCCCCC ACAGCGAGGT GTTCCCCGAG
     1561 CTGCTGCGCG AGCGCCGCGC CGCCACCGTG GAGGACCACC GCGGCTGGGG CTACCTGTCC
     1621 CCGCGCCTGC TGGACCGCCT GGAGGACTGG CTGCCCGACG TGGACACCCT GCTGGCCGGC
     1681 CGCGAGCCCC GCTTCGTGCA CGGCGACCTG CACGGCACCA ACATCTTCGT GGACCTGGCC
     1741 GCGACCGAGG TGACCGGCAT CGTGGACTTC ACCGACGTGT ACGCCGGCGA CAGCCGCTAC
     1801 AGCCTGGTGC AGCTGCACCT GAACGCCTTC CGCGGCGACC GCGAGATCCT GGCGGCCCTG
     1861 CTGGACGGCG CCCAGTGGAA GCGCACCGAG GACTTCGCCC GCGAGCTGCT GGCCTTCACC
     1921 TTCCTGCACG ACTTCGAGGT GTTCGAGGAG ACCCCCCTGG ACCTGAGCGG CTTCACCGAC
     1981 CCCGAGGAGC TGGCCCAGTT CCTGTGGGGC CCCCCCGACA CCGCCCCCGG CGCCTGATAA
     2041 CATATGGGGT CTAGAGAATT CGGATCCCCG CTCCGTGTAA ATGGAGGCGC TCGTTGATCT
     2101 GAGCCTTGCC CCCTGACGAA CGGCGGTGGA TGGAAGATAC TGCTCTCAAG TGCTGAAGCG
     2161 GTAGCTTAGC TCCCCGTTTC GTGCTGATCA GTCTTTTTCA ACACGTAAAA AGCGGAGGAG
     2221 TTTTGCAATT TTGTTGGTTG TAACGATCCT CCGTTGATTT TGGCCTCTTT CTCCATGGGC
     2281 GGGCTGGGCG TATTTGAAGC GGGTACCCAG CTTTTGTTCC CTTTAGTGAG GGTTAATTGC
     2341 GCGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT
     2401 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG
     2461 CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG
     2521 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC
     2581 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC
     2641 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA
     2701 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
     2761 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     2821 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     2881 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     2941 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     3001 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     3061 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG
     3121 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC
     3181 TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC
     3241 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG
     3301 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT
     3361 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT
     3421 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA
     3481 ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA
     3541 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT
     3601 GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG
     3661 AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA
     3721 GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA
     3781 AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG
     3841 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC
     3901 AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC
     3961 GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA
     4021 TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC
     4081 CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG
     4141 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC
     4201 GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG
     4261 TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC
     4321 AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT
     4381 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA
     4441 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA
     4501 AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG
     4561 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC
     4621 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG
     4681 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC
     4741 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
     4801 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC
     4861 TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA
     4921 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTT
//
