LOCUS       pAPHVIII_pPH75      4602 bp    DNA     circular SYN 18-SEP-2014
DEFINITION  Marker plasmid for C. reinhardtii transformation with paromomycin selection. Design by Irina Sizova. Deposited by the Hegemann lab (contact: Simon Kelterborn; sk@chlamy.de; www.chlamy.de)
ACCESSION   .
VERSION     .
DBSOURCE    GenBank
KEYWORDS    APHVIII, C.reinhardtii
REFERENCE   1  
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Montag, 12. Mai 2014 from SnapGene 2.3.5 http://www.snapgene.com
FEATURES             Location/Qualifiers
     reporter        1..160
                     /label="lacZ-a"
     primer_bind     116..135
                     /note="Primer: CCAGGGTTTTCCCAGTCACG"
                     /label="M13-24F"
     primer_bind     140..157
                     /note="Primer: TGTAAAACGACGGCCAGT"
                     /label="M13-21F"
     mutation        160..162
                     /label="GCGCGC -> ATT"
     primer_bind     164..182
                     /note="Primer: TAATACGACTCACTATAGG"
                     /label="T7prom"
     promoter        219..508
                     /label="HSP70A"
     mutation        308
                     /label="C->G"
     promoter        509..729
                     /label="RBCS2"
     CDS             join(730..737,883..1696)
                     /label="APHVIII(v2)"
     intron          738..882
                     /label="RBCS2 Intron"
     terminator      1703..1936
                     /label="RBCS2"
     primer_bind     complement(1954..1973)
                     /note="Primer: AATTAACCCTCACTAAAGGG"
                     /label="T3"
     primer_bind     complement(1993..2010)
                     /note="Primer: CAGGAAACAGCTATGACC"
                     /label="M13-29R"
     primer_bind     complement(2007..2027)
                     /note="Primer: CGGATAACAATTTCACACAGG"
                     /label="M13-24R"
     rep_origin      2381..3000
                     /label="pBR322 ori (high copy)"
     CDS             complement(3155..4015)
                     /product="beta-lactamase"
                     /note="AmpR confers resistance to ampicillin,
                     carbenicillin, and related antibiotics"
                     /label="AmpR (bla)"
     promoter        complement(4016..4120)
                     /label="AmpR"
     rep_origin      4147..4602
                     /note="f1 ori f1 bacteriophage origin of replication;
                     arrow  indicates direction of (+) strand synthesis"
                     /label="f1 origin"
ORIGIN
        1 CCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT
       61 ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG
      121 GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGAA TTGTAATACG ACTCACTATA
      181 GGGCGAATTG GAGCTCCACC GCGGTGGCGG CCGCTCTAGA GACGGCGGGG AGCTCGCTGA
      241 GGCTTGACAT GATTGGTGCG TATGTTTGTA TGAAGCTACA GGACTGATTT GGCGGGCTAT
      301 GAGGGCGGGG GAAGCTCTGG AAGGGCCGCG ATGGGGCGCG CGGCGTCCAG AAGGCGCCAT
      361 ACGGCCCGCT GGCGGCACCC ATCCGGTATA AAAGCCCGCG ACCCCGAACG GTGACCTCCA
      421 CTTTCAGCGA CAAACGAGCA CTTATACATA CGCGACTATT CTGCCGCTAT ACATAACCAC
      481 TCAGCTAGCT TAAGATCCCA TCAAGCTTGC ATGCCGGGCG CGCCAGAAGG AGCGCAGCCA
      541 AACCAGGATG ATGTTTGATG GGGTATTTGA GCACTTGCAA CCCTTATCCG GAAGCCCCCT
      601 GGCCCACAAA GGCTAGGCGC CAATGCAAGC AGTTCGCATG CAGCCCCTGG AGCGGTGCCC
      661 TCCTGATAAA CCGGCCAGGG GGCCTATGTT CTTTACTTTT TTACAAGAGA AGTCACTCAA
      721 CATCTTAAAA TGGCCAGGTG AGTCGACGAG CAAGCCCGGC GGATCAGGCA GCGTGCTTGC
      781 AGATTTGACT TGCAACGCCC GCATTGTGTC GACGAAGGCT TTTGGCTCCT CTGTCGCTGT
      841 CTCAAGCAGC ATCTAACCCT GCGTCGCCGT TTCCATTTGC AGGATGCTCG AGATGGACGA
      901 CGCTCTGCGG GCTCTGCGGG GCCGGTACCC TGGCTGCGAG TGGGTGGTGG TGGAGGACGG
      961 CGCCTCTGGC GCTGGCGTGT ACCGCCTGCG CGGTGGCGGC CGCGAGCTGT TCGTGAAGGT
     1021 GGCGGCCCTG GGTGCGGGTG TGGGTCTGCT CGGCGAGGCG GAGCGCCTCG TGTGGCTGGC
     1081 CGAGGTCGGC ATCCCCGTCC CGCGCGTGGT CGAGGGTGGC GGCGACGAGC GCGTGGCGTG
     1141 GCTGGTCACG GAGGCCGTGC CTGGCCGCCC AGCTAGCGCA CGGTGGCCCC GCGAGCAGCG
     1201 CCTGGACGTC GCCGTGGCGC TGGCTGGCCT GGCGCGCAGC CTGCACGCTC TGGACTGGGA
     1261 GCGCTGCCCC TTCGACCGGT CGCTGGCCGT GACTGTCCCG CAAGCTGCTC GCGCCGTGGC
     1321 CGAGGGCTCC GTGGACCTGG AGGACCTGGA TGAGGAGCGC AAGGGCTGGT CTGGCGAGCG
     1381 CCTGCTCGCG GAGCTGGAGC GTACCCGCCC CGCGGACGAG GATCTGGCCG TGTGCCACGG
     1441 CGACCTGTGT CCCGATAACG TGCTGCTGGA CCCCCGCACC TGCGAGGTGA CGGGCCTGAT
     1501 TGACGTGGGT CGTGTGGGTC GTGCGGACCG GCACTCAGAC CTGGCCCTCG TGCTGCGCGA
     1561 GCTGGCGCAT GAGGAGGACC CCTGGTTCGG CCCTGAGTGC TCGGCCGCCT TCCTGCGCGA
     1621 GTATGGCCGG GGCTGGGACG GCGCTGTGTC TGAGGAGAAG CTGGCGTTCT ACCGGCTGCT
     1681 GGACGAGTTC TTCTGAGGAT CCCCGCTCCG TGTAAATGGA GGCGCTCGTT GATCTGAGCC
     1741 TTGCCCCCTG ACGAACGGCG GTGGATGGAA GATACTGCTC TCAAGTGCTG AAGCGGTAGC
     1801 TTAGCTCCCC GTTTCGTGCT GATCAGTCTT TTTCAACACG TAAAAAGCGG AGGAGTTTTG
     1861 CAATTTTGTT GGTTGTAACG ATCCTCCGTT GATTTTGGCC TCTTTCTCCA TGGGCGGGCT
     1921 GGGCGTATTT GAAGCGGGTA CCCAGCTTTT GTTCCCTTTA GTGAGGGTTA ATTGCGCGCT
     1981 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC
     2041 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC
     2101 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC
     2161 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG
     2221 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
     2281 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
     2341 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
     2401 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
     2461 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
     2521 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
     2581 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
     2641 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
     2701 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
     2761 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
     2821 ACGGCTACAC TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
     2881 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
     2941 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
     3001 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
     3061 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
     3121 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC
     3181 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
     3241 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC
     3301 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
     3361 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA
     3421 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG
     3481 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
     3541 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG
     3601 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
     3661 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT
     3721 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA
     3781 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
     3841 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC
     3901 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
     3961 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT
     4021 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
     4081 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
     4141 CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG
     4201 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC
     4261 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC
     4321 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA
     4381 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA
     4441 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG
     4501 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA
     4561 AATTTAACGC GAATTTTAAC AAAATATTAA CGCTTACAAT TT
//
