Humboldt-Universität zu Berlin - GENOME EDITING WITH CRISPR 2019

Scientific Program




Genome Editing with CRISPR 

September 4 - 6, 2019

Humboldt-Universität zu Berlin






Annual conference of the German Genetics Society:




WEDNESDAY, September 4, 2019


12.00 – 14.00 Registration


14.00             Opening remarks: Ann Ehrenhofer-Murray



SESSION: “Class2” CRISPR-Cas systems

Chair: Ann Ehrenhofer-Murray


14.15 – 15.00     KEYNOTE LECTURE:

                         Emmanuelle Charpentier, MPI for Infection Biology, Berlin



15.00 – 15.30     Virginijus Siksnys, Vilnius University, Institute of

                          Biotechnology Lithuania

                          Mining CRISPR-Cas systems: from Cas9s to auxiliary proteins


15.30 – 16.00     Dipali Sashital, Iowa State University, Ames, IA

                         Pervasive off-target and double-stranded DNA nicking by



16.00 - 16.30     Chase Beisel, Helmholtz Institute for RNA-based Infection

                         Research (HIRI), Würzburg

                         Characterization of Cas12a nucleases reveals diverse PAM

                         profiles between closely-related orthologs


Coffee break


SESSION: “Class 1” systems

 Chair: Emmanuelle Charpentier


17.00 – 17.30     Peter Fineran, University of Otago, New Zealand

                         The regulation and activity of Class 1 CRISPR-Cas systems


17.30 – 18.00     Malcom White, St. Andrews University, UK

                          Ring War: virus:host conflict involving type III CRISPR



18.00 – 18.30     John van der Oost, University Wageningen, NL

                         The Cascade complex - from biology to applications


18.30 – 19.00    Lennart Randau, Max-Planck-Institute for Terrestrial

                        Microbiology, Marburg

                        Structural and functional insights into Type IV 

                        CRISPR-Cas systems


19.00                 Welcome reception and Poster Session I



THURSDAY, September 5, 2019

SESSION: Anti-CRISPR proteins

Chair: Malcolm White


9.00 – 9.30     Karen L. Maxwell, University of Toronto, Canada

                      The role of Anti CRISPR in the phage-host evolutionary

                      arms race


9.30 – 10.00   Joe Bondy-Denomy, UCSF

                     Evasion of CRISPR nucleases by jumbo bacteriophages


10.00 – 10.20 Dimitrios Laurin Wagner, Charité Universitätsmedizin Berlin

                     T cell immunity toward CRISPR-associated nucleases


Coffee break


SESSION:CRISPR-Cas technology and applications

Chair: Chase Beisel



10.45 – 11.15 Alekos Simoni, Imperial College London, UK

                     CRISPR-based population editing for vector control


11.15 – 11.45 Holger Puchta, Karlsruhe Institute of Technology,   


                      CRISPR/Cas mediated genome engineering: a revolution for

                      plant biology and breeding


11.45 – 12.05 Cornelia Hampe, TaKaRa Bio Europe

                      A fast and reliable method for SNP screening in genome

                      editing experiments


12.05 – 12.25 Chunyu Liao, Helmholtz-Zentrum für RNA-basierte

                      Infektionsforschung, Würzburg

                      Modular one-pot assembly of CRISPR arrays enables

                      library generation and reveals factors influencing crRNA



Lunch break


13.00 – 13.30 Gesellschaft für Genetik Members Meeting


13.30 – 16.00 Speed talks and Poster Session II


SESSION: Adaptation

Chair: Lennart Randau


16.00 – 16.30 Francisco Mojica, University of Alicante, Spain 

                     Determinants of CRISPR adaptation preferences, still

                     an open issue 


16.30 – 17.00 Stan J. J. Brouns, TU Delft, NL

                     How microbes keep their CRISPR memories functional and

                     up to date


17.00 – 17.30 Anita Marchfelder, Universität Ulm, Germany

                     Self-targeting induced adaptation


17.30 – 17.50 Minja Velimirovic, Universite Laval/CHUL, Quebec

                      Increasing the genome-targeting scope of base editing using 

                      Streptococcus thermophilus CRISPR1-Cas9 system


17.50 – 18.10 Daphne Collias, Helmholtz-Zentrum für RNA-basierte

                     Infektionsforschung (HIRI), Würzburg

                     A positive, growth-based PAM screen reveals non-canonical     

                     motifs recognized by the S. pyogenes Cas9


18.10 – 18.30 Edda Schulz, Max Planck Institute for Molecular 

                     Genetics, Berlin

                     A hierarchical CRISPR screening approach to identify genes 

                     underlying sex differences in mouse embryonic stem cells 


19.00              Conference Dinner: 

                      HABEL am Reichstag, Luisenstraße 19, 10117 Berlin



FRIDAY, September 6, 2019


SESSION: CRISPR-Cas functions beyond defense

Chair: Stan Brouns


 9.00 – 9.30    Eugene V. Koonin, National Center for Biotechnology

                      Information, NIH, Bethesda, MD

                      Diversity, evolution, and exaptation of CRISPR-Cas systems


9.30 – 10.00   Cynthia Sharma, Universität Würzburg

                      Targeting of endogenous RNAs by the CRISPR-Cas9 

                      nuclease in Campylobacter jejuni


10.00 – 10.20  Ed Bolt, University of Nottingham, UK

                      A new sgRNA– targeted DNA integration protein for genome 

                      editing that short – circuits homology dependent DNA repair


Coffee break

SESSION: Prizes and Awards

Chair: Ann Ehrenhofer-Murray


10.45 – 11.45 MAX-DELBRÜCK-LECTURE: 

                      Detlef Weigel, MPI für Entwicklungsbiologie, Tübingen

                      Epistasis, the spice of life: Lessons from the study of the

                      plant immune system


11.45 – 12.30 GATEFF Award for Excellent PhD Thesis



                   CELEBRATION: Honorary Membership for Prof. Elisabeth Gateff


Lunch break


13.30 – 14.30 Poster Session III


SESSION: Ecology & Evolution

Chair: Peter Fineran



14.30 – 15.00 Uri Gophna, Tel Aviv University, Israel

                     CRISPR-Cas as a moderator of the flexible genome


15.00 – 15.30 Edze Westra, University of Exeter, UK

                     Mixed mode transmission of phages drives loss of Type

                     I CRISPR-Cas immune systems from bacteria


15.30 – 15.50 Israela Turgeman-Grott, Tel Aviv University

                     The host, the CRISPR and the integrated virus


15.50 – 16.10 Stanislav Indik, University of Veterinary Medicine


                      A novel toolkit for the efficient delivery of Cas9/sgRNA 

                      complexes to cells


16.15 Concluding remarks and departure




                     (MUSEUM FÜR NATURKUNDE, Invalidenstr. 43,  

                     10117 Berlin)

                    „Genomeditierung - Wo sind die Grenzen?“

                    Public event with Detlef Weigel (MPI für Entwicklungsbiolgie,

                    Tübingen) and Sheree Domingo (artist) 

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