Humboldt-Universität zu Berlin - Experimentelle Biophysik/Mechanobiologie (Juniorprofessur)

Selected Publications

1. Wagner DL§, Klotzsch E.§ (2022), Barring the gates to the battleground: DDR1 promotes immune exclusion in solid tumors, Nature Sig. Transd. and Targ. TherapyWe highlighted a paper Sun et al. 2021, Nature, that shows antibodies against DDR1 to facilitate solid tumor invasion,


2. Aramesh M§, Stoycheva D, Sandu I, Ihle S, Zünd T, Shiu J, Forro C, Asghari M, Bernero M, Lickert S, Oxenius A, Vogel V, Klotzsch E.§ (2021), Nanoconfinement of Microvilli Alters Gene Expression and Boosts T cell Activation. Proc. Natl. Acad. Science U.S.A., 118 (40) e2107535118; Here we use nanostructured anodized aluminium oxide to boost T-cell activation and proliferation. The large extracellular domain of CD45 leads to an artificially induced separation from the TCR which leads to T cell activation even without antibodies.


3.Emmanouilidis L, Esteban-Hofer L, Damberger F, de Vries T, Nguyen C, Fábregas Ibáñez I, Mergenthal S, Klotzsch E, Yulikov M, Jeschke G and Allain F  (2021) NMR and EPR reveal a compaction of the RNA-binding protein FUS upon droplet formation. Nature Chem. Biol., In collaboration with colleagues at ETH Zurich, we looked at NTD FUS aggregation.


4 . Stoycheva D, Simsek H, Weber W, Hauser A and Klotzsch E§  (2021) External cues to drive B cell function towards immunotherapy. microscope. Acta Biomaterialia, Here we review literature of B cell function in the context of tumor immunology.


5. Aramesh M, Mergenthal S, Issler M, Plochberg B, Weber F, Qin XH, Liska R, Huppa J, Duda GN, Huppa JB, Ries J, Schuetz GJ§ and Klotzsch E§  (2021) Functionalized Bead Assay to Measure Three-dimensional Traction Forces during T-cell Activation. microscope. Nano Letters, We developed a new platform to measure traction forces of activating T cells.


6. Aramesh M, Forró C, Dorwling-Carter L, Lüchtefeld I, Schlotter T, Ihle SJ, Shorubalko I, Hosseini V, Momotenko D, Zambelli T, Klotzsch E, Vörös J§ (2019) Localized detection of ions and biomolecules with a force-controlled scanning nanopore microscope. Nature Nano., Using nanopore tips on an AFM we detect single molecule secretion through cellular channels.


7. Karner A, Nimmervoll B, Plochberger B, Klotzsch E, Horner A, Knyazev D, Kuttner R, Winkler K, Winter L, Siligan C, Ollinger N, Pohl P, Preiner J (2016) Tuning membrane protein mobility by confinement into nanodomains. Nature Nano.,  Using high speed AFM and fluorescence single particle tracking we looked at artificial lipid bilayers to tune single transmembrane proteins confinement.


8. Anderluh A,  Hofmaier T, Klotzsch E, Kudlacek O, Stockner T, Sitte HH, Schütz GJ (2017)  Direct PIP2 binding mediates stable oligomer formation of the serotonin transporter. Nature Comm., Combining single molecule analysis and molecular modeling, we observe how serotonin transporters form oligomers on the cellular membrane.


9. Klotzsch E§,*, Smorodchenko A*, Löfler L, Parkinson E, Moldzio R, Schütz GJ§, Pohl EE§ (2015), Super-resolution microscopy reveals the spatial separation of UCP4 and F0F1-ATP synthase in neuronal mitochondria. Proc. Natl. Acad. Science U.S.A., in press, 14 citations. Using two color super-resolution microscopy of utmost importance to this project, this study shows that UCP4 and F0F1ATPase, competing for proton gradients, are spatially separated at the inner mitochondrial membrane in neurons.


10. Kubow KE, Vuikmirovic R, Zhe L, Klotzsch E, Smith ML, Gourdon D, Luna S, Vogel V§ (2015), Mechanical forces regulate the interactions of fibronectin and collagen I in extracellular matrix. Nature Comm., in press, 17 citations. Using FRET and binding assays we  measured the mechanical regulated interplay of the two extracellular matrix components fibronectin and collagen.


11. Anderluh A, Klotzsch E§, Reismann AWAF, Brameshuber M, Kudlacek O, Newman AH, Sitte HH and Schütz GJ§ (2014), Single Molecule Analysis Reveals Coexistence of Stable Serotonin Transporter Monomers and Oligomers in the Live Cell Plasma Membrane, J.Biol.Chem. 289, 4387-4394. This study describes the role of oligomeric states of the serotonin transporters within the plasma membrane. The results reveal the first evidence for kinetic trapping of preformed neurotransmitter transporter oligomers.


12. Anderluh A, Klotzsch E§, Ries J, Reismann AWAF, Weber S, Fölser M, Koban F, Freissmuth M, Sitte HH and Schütz GJ§ (2014), Tracking Single Serotonin Transporter Molecules at the Endoplasmic Reticulum and Plasma Membrane. Biophys J. 106, L33-L35. This study shows different modes of diffusion of the serotonin transporter within the ER and the plasma membrane potentially due to interaction with the cytoskeleton.


13. Klotzsch E, Schön I, Ries J, Renn A, Sandoghdar V & Vogel V. § (2014), Single Molecule Localization Microscopy With Multiple Fluorophores To Explore the Conformation of Surface-Adsorbed Fibronectin Molecules. Biomaterials Science. 2, 883-892, IF=4.5, 9 citations. This study shows, that the conformation of single molecules can be altered by solution and surface conditions, for that I built a single molecule setup.


14. Klotzsch E, Schütz GJ (2013), Improved ligand-discrimination by force-induced unbinding of the T cell receptor from peptide-MHC. Biophys J. 104 (8), 1670-1675, IF=3.7, 7 citation. In this article we introduce a theoretical model of forces improving the potency of ligand discrimination in the case of t-cell receptor – peptide MHC specificity, this is the theoretical foundation of force mediated T-cell activation.


15. Schoen I*, Ries J*, Klotzsch E, Ewers H§ & Vogel V§ (2011) Binding-Activated Localization Microscopy of DNA Structures. Nano Lett. 11 (9), 4008-4011, IF=13, 66 citations. This study introduces a new super-resolution technique, that can be used to significantly improve labeling density without losing localization precision.


16. Klotzsch E*, Smith ML*, Kubow KE, Muntwyler S, Little WC, Beyeler F, Gourdon D, Nelson BJ, & Vogel V§ (2009) Fibronectin forms the most extensible biological fibers displaying switchable force-exposed cryptic binding sites. Proc Natl Acad Sci U S A 106, 18267-18272., IF=9.7, 137 citations. This study for the first time uses FRET as an optical readout to measure stress induced conformational change, hence exposure of cryptic epitopes that can influence the cells fate. This is the key paper of my PhD thesis and my entry in the world of mechanobiology.


* equal contribution, § correspondence


full list can be found here: Google Scholar