Humboldt-Universität zu Berlin - Experimentelle Biophysik



  • Master- oder Doktorarbeiten

Masters Project Studentship available on Expression and Purification of Enzymerhodopsins


A Masters Project Studentship is available in the research group of Prof. Peter Hegemann in the Institute for Biology, Experimental Biophysics, Humboldt University of Berlin. This is an exciting opportunity to work on a project involving the expression and purification of Enzymerhodopsins that show light-regulated enzyme activity. The cyclic nucleotides cAMP and cGMP are ubiquitous second messengers, known for regulating several crucial cellular processes, including, basic metabolism, gene expression, differentiation, and cell survival. In this project, we shall characterize the photophysical and structural properties of the rhodopsin-guanylyl cyclase (RhGC) that produces cGMP in response to green light, from either Catenaria anguillulae or Blastocladiella emersonii. Specifically, we wish to study the structure of these proteins using cryo-EM technique. The structure and properties of the corresponding adenylyl cyclases (CaRhACs) will also be studied as these photoreceptors proteins have great potential for application in optogenetics and possess multiple advantages over the currently existing tools to manipulate cyclic-nucleotide dynamics in living cells. The candidate should have a Bachelors degree in Biophysics, Biochemistry, Biology or a related area. Experience in protein expression and purification is advangage but not essencial. For details, please contact Dr. Ramprasad Misra via e-mail ID ramprasad.misra(at)

(1) U. Scheib et al., Rhodopsin-cyclases for photocontrol of cGMP/cAMP and 2.3 Å structure of the adenylyl cyclase domain. Nature Communications 2018, 9, Article number: 2046. 
(2) S. Mukherjee, M. Broser and P. Hegemann, Enzymerhodopsins: novel photoregulated catalysts for optogenetics. Current Opinion in Structural Biology 2019, 57, 118-126.


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  • Wissenschaftliche Mitarbeiter (m/w/d)

The Cluster of Excellence “Neurocure” offers a fixed term position as Researcher / PostDoc. Subject to personal qualification employees are remunerated to salary E 13 TV-L HU

Starting 01.01.2020, duration: 21 months

Research Area:

Molecular characterization and improvement of bimodal switchable Opto-GPCRs used for the activation of G-alpha signaling in central neurons.

The project will includer structure-based design, medium size screening methods, electrophysiology, biochemistry, spectroscopy and high-resolution imaging.

Qualification profile:

We aim at attracting the best talent in the respective research fields and look for:

• an outstanding university degree (master/diploma or equivalent) and Dr. or PhD degrees in biochemistry, chemistry, physics, biology, or related field of physical sciences

• Dr. or PhD degree from an excellent research group / University

• strong knowledge in biochemistry or biophysics

• an independent, target- and solution-driven work attitude

• multidisciplinary thinking,

• strong motivation and interest to join one of the most ambitious interdisciplinary research clusters,

• very good interpersonal and communication skills; in particular, the ability to effectively work in collaborative research efforts,

• fluency in English - written and oral.

We offer:

You will join a team of enthusiastic scientists who pursue creatively their individual research project inspired by the cluster’s innovative approach and support. We will offer connection to a large number of outstanding research groups within the currently most encouraging city of Berlin.

• access to state of the art research of leading academic institutes.

• multidisciplinary education

• International Doctoral / PostDoctoral program

• promotion of gender equality and family-friendly work environment. Send application to Applications from women are particularly welcome. The same applies to people with disabilities.

Your electronic application (in English only) should be a single pdf-file including: motivation letter, CV, copy of degree certificate, transcript of grades and proof of English language skills.

Complete applications should be submitted to Dr. Wiebke Apel: quoting the reference number Kennziffer DR / 154 / 19 in the subject header to

The closing date for applications is 31.10.2019.