Humboldt-Universität zu Berlin - AG Zelluläre Biophysik

Publications from the group


The group now routinely submits all finished work to bioRXiv or a similar server, usually directly sending the preprint for peer review at the same time. Our experience to date (since 2017) is entirely positive.

State-dependent photocrosslinking unveiled the role of intersubunit interfaces on Ca2+activating mechanisms of the BK channel

Alberto J. Gonzalez-Hernandez, Belinda Rivero-Pérez, David Bartolomé-Martín, Diego Álvarez de la Rosa, Andrew J.R. Plested, Teresa Giraldez

Peer reviewed articles and commentaries


An optogenetic method for the controlled release of single molecules.

Kashyap P, Bertelli S, Cao F, Kostritskaia Y, Blank F, Srikanth NA, Schlack-Leigers C, Saleppico R, Bierhuizen D, Lu X, Nickel W, Campbell RE, Plested AJR, Stauber T, Taylor MJ, Ewers H.
doi: 10.1038/s41592-024-02204-x. PMID: 38459384.


Controlling the interaction between CaMKII and Calmodulin with a photocrosslinking unnatural amino acid.

Lučić I, Jiang PL, Franz A, Bursztyn Y, Liu F, Plested AJR
doi: 10.1002/pro.4798.

CaMKII autophosphorylation can occur between holoenzymes without subunit exchange

Iva Lučić, Léonie Héluin, Pin-Lian Jiang, Alejandro G Castro Scalise, Cong Wang, Andreas Franz, Florian Heyd, Markus Wahl, Fan Liu, Andrew J R Plested
doi: 10.7554/eLife.86090

Mechanism of Calcium Permeation in a Glutamate Receptor Ion Channel

FK Schackert, J Biedermann, S Abdolvand, S Minniberger, C Song, AJR Plested, P Carloni, and H Sun
doi: 10.1021/acs.jcim.2c01494

Asymmetry and ion selectivity properties of bacterial channel NaK mutants derived from ionotropic glutamate receptors

S Minniberger, S Abdolvand, S Braunbeck, H Sun, AJR Plested
doi: 10.1016/j.jmb.2023.167970

Targeted sensors for glutamatergic neurotransmission
Y Hao, E Toulmé, B König, C Rosenmund, AJR Plested
doi: 10.7554/eLife.84029


Calcium-permeable channelrhodopsins for the photocontrol of calcium signalling

Rodrigo G Fernandez Lahore, Niccolò P Pampaloni, Enrico Peter, M-Marcel Heim, Linda Tillert, Johannes Vierock, Johannes Oppermann, Jakob Walther, Dietmar Schmitz, David Owald, Andrew JR Plested, Benjamin R Rost, Peter Hegemann
doi: 10.1038/s41467-022-35373-4

Seeing glutamate at central synapses  (Review)
Y Hao, AJR Plested
Journal of Neuroscience Methods 375, 109531

Selective endocytosis of Ca2+-permeable AMPARs by the Alzheimer’s disease risk factor CALM bidirectionally controls synaptic plasticity
Domenico Azarnia Tehran, Gaga Kochlamazashvili, Niccolò P Pampaloni, Silvia Sposini, Jasmeet Kaur Shergill, Martin Lehmann, Natalya Pashkova, Claudia Schmidt, Delia Löwe, Hanna Napieczynska, Arnd Heuser, Andrew JR Plested, David Perrais, Robert C Piper, Volker Haucke, Tanja Maritzen
doi: 10.1126/sciadv.abl5032

The action of Con-ikot-ikot toxin on single AMPA-type glutamate receptors
J Baranovic, S Braunbeck, N Zaki, S Minniberger, M Chebli, AJR Plested
doi: 10.1085/jgp.202112912

Crosslinking glutamate receptor ion channels
Andrew J.R. Plested, Mette H.Poulsen
doi: 10.1016/bs.mie.2021.03.005

Slow excitatory synaptic currents generated by AMPA receptors (Symposium Review)
Niccolò Paolo Pampaloni & Andrew J. R. Plested
doi: 10.1113/JP280877


Slow AMPA receptors in hippocampal principal cells
Niccolò Paolo Pampaloni, Irene Riva, Anna Carbone, Andrew J. R. Plested
doi: 10.1016/j.celrep.2021.109496

Glutamate Receptor complexes open up and reveal their molecular dialect (News and Views)
Andrew J.R. Plested
doi: 10.1038/d41586-021-01380-6

Non-selective cation permeation in an AMPA-type glutamate receptor
Johann Biedermann, Sebastian Braunbeck, Andrew J. R. Plested, Han Sun
doi: 10.1101/2020.06.21.162735

NMDA Receptors Singled out for Delayed Activation (Preview)
Andrew J.R.Plested
doi: 10.1016/j.neuron.2021.01.012


Measurements of the Timescale and Conformational Space of AMPA Receptor Desensitization
Salazar H, Mischke S, Plested AJR.
PMID: 32559412


Gating modules of the AMPA receptor pore domain revealed by unnatural amino acid mutagenesis.
Poulsen MH, Poshtiban A, Klippenstein V, Ghisi V, Plested AJR.
PMID: 31213549

A FRET sensor of C-terminal movement reveals VRAC activation by plasma membrane DAG signaling rather than ionic strength.
König B, Hao Y, Schwartz S, Plested AJ, Stauber T.
PMID: 31210638 


Auxiliary Proteins keep AMPA receptors compact during activation and desensitization
Baranovic J and Plested AJR

AMPA receptors - mechanisms of auxiliary protein action
Eibl C and Plested AJR

Neurotransmitter Funneling Optimizes Glutamate Receptor Kinetics
Yu A, Salazar H, Plested AJR, Lau AY


Control of AMPA receptor activity by the extracellular loops of auxiliary proteins
Riva I, Eibl C, Volkmer R, Carbone AL, Plested AJR

Unitary Properties of AMPA Receptors with Reduced Desensitization
Zhang W, Eibl C, Weeks AM, Riva I, Li Y-J, Plested AJR, Howe JR

Mechanism of partial agonism in AMPA-type glutamate receptors
Salazar H, Eibl C, Chebli M, Plested AJR


Single-Channel Recording of Ligand-Gated Ion Channels.
Plested AJ.
Cold Spring Harb Protoc.  doi: 10.1101/pdb.top087239. PMID: 27480725

Single-Channel Recording of Glycine Receptors in Human Embryonic Kidney (HEK) Cells.
Plested AJ, Baranovic J.
Cold Spring Harb Protoc. doi: 10.1101/pdb.prot091652. PMID: 27480721

Structural rearrangement of the intracellular domains during AMPA receptor activation
Zacchariassen LG, Katchan L, Jensen AG, Pickering DS, Plested AJR & Kristensen AS 
PNAS doi/10.1073/pnas.1601747113

Structural mechanisms of activation and desensitisation in neurotransmitter-gated ion channels. 
Plested AJR
NSMB 23, 494–502

Dynamics of the ligand binding domain layer during AMPA receptor activation
Baranovic J, Chebli M, Salazar H, Carbone AL, Faelber K, Lau AY, Daumke O, Plested AJR
Biophysical Journal

Superactivation of AMPA Receptors by Auxiliary Proteins
Carbone AL and Plested AJR
Nature Communications doi:10.1038/ncomms10178


How to build the fastest receptor on earth.
Baranovic J, Plested AJ. Review  
Biol. Chem.  doi: 10.1515/hsz-2015-0182. 


Gating characteristics control Glutamate Receptor distribution and trafficking in vivo
Petzoldt AG, Lee Y-H, Khorramshahi O, Reynolds E, Plested AJR, Herzel H and Sigrist SJ 
Current Biology doi: 10.1016/j.cub.2014.07.051

Don't Flip Out: AChRs are primed to Catch and Hold Your Attention
Plested, AJR
Biophysical Journal Volume 107 , Issue 1 , 8 - 9

Photoinactivation of glutamate receptors by genetically encoded unnatural amino acids.
Klippenstein V, Ghisi V, Wietstruk M, Plested AJ.
J Neurosci. 34(3):980-91. doi: 10.1523/JNEUROSCI.3725-13.2014.


A Conformational Intermediate in Glutamate Receptor Activation
Lau AY, Salazar H, Blachowicz L, Ghisi V, Plested AJ, Roux B.
Neuron. Volume 79 Issue 3 492-503. doi: 10.1016/j.neuron.2013.06.003.

State-dependent FRET reports calcium- and voltage-dependent gating-ring motions in BK channels
Pablo Miranda, Jorge E. Contreras, Andrew J. R. Plested, Fred J. Sigworth, Miguel Holmgren and Teresa Giraldez
PNAS doi:10.1073/pnas.1219611110


Coupled Control of Desensitization and Gating by the Ligand Binding Domain of Glutamate Receptors
Carbone AL and Plested AJR
Neuron Volume 74 Issue 5, 845-857, 7 June 

The α1K276E Startle Disease Mutation Reveals Multiple Intermediate States in the Gating of Glycine Receptors
Lape R, Plested AJR, Moroni M, Colquhoun D, Sivilotti LG 
The Journal of Neuroscience,  doi: 10.1523/ JNEUROSCI.4346-11.2012


Kainate Receptor Modulation by Sodium and Chloride
Plested AJR in Kainate Receptors: Novel Signalling Insights
Edited by Rodríguez-Moreno, A. and Sihra, T.   Landes Bioscience


Domain Organization and Function in GluK2 subtype Glutamate Receptors
Das UT, Kumar J, Mayer ML and Plested AJ. 
PNAS  doi: 10.1073/pnas.1000838107


How to button a bouton with alpha(2)deltas
Sigrist SJ, Plested AJ.
Nature Neuroscience 12(11):1357-8.