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Humboldt-Universität zu Berlin - Experimentelle Biophysik

Humboldt-Universität zu Berlin | Institut für Biologie | Experimentelle Biophysik | Home | Chlamydomonas | Strains deposited by the Hegemann lab at the Chlamydomonas Resource Center

Strains deposited by the Hegemann lab at the Chlamydomonas Resource Center

Visit www.chlamy.de for more info or contact CRISPR@chlamy.de. Please verify your strains by sequencing of the target region.

 

Photoreceptor Knock-outs

 

COP1/2

Chlamyopsin (COP1/2) disruption strains.

in CC-125: CC-5398 ∆cop1/2-F4 [PH77], CC-5399cop1/2-F7 [PH78]

 

ChR1 (COP3)

Channelrhodopsin 1 (ChR1) disruption strains:

in CC-3403:CC-5546 ∆chr1-M6-F4-3 [PH167], CC-5547 ∆chR1-E12 [PH166]

in SAG 11-32b: CC-5550 ∆chR1-H6 mt+ [PH160], CC-5551 ∆chR1-F1 mt+ [PH163]

 

ChR2 (COP4)

Channelrhodopsin 2 (ChR2) disruption strains:

in CC-3403: CC-5548 ∆chR2-F12 mt- [PH162], CC-5549 ∆chR2-D11 mt- [PH161], 

in SAG 11-32b: CC-5552 ∆chR2-H9 mt+ [PH165], NA ∆chR2-H9 mt+ [PH164]

 

ChR1 ChR2

Channelrhodopsin 1+2 double Knock-out strains:

in CC-125: CC-5499 ∆ChR1 ∆ChR2 [PH128], 

 

COP5 (HKR1)

Chlamyopsin 5 (COP5, HKR1) disruption strains.

in CC-3403: CC-5437cop5.20-B2 [PH042], CC-5438cop5.12-H3 [PH043]

in CC-125: CC-5443cop5-D11 [PH065], CC-5448cop5-E6 [PH103]

 

COP6 (HKR2)

Chlamyopsin 6 (COP6, HKR2) disruption strains.

in CC-125: CC-5431cop6-F9 [PH143], CC-5432cop6-G4 [PH142]

 

COP8 (HKR4)

Chlamyopsin 8 (COP8, HKR4) disruption strains.

in CC-3403: CC-5425cop8-E9D1 [PH101], CC-5426cop8-F1A1 [PH102]

 

COP11/12 (HKR7/8)

Chlamyopsin 11/12 (COP11/12, HKR7/8) disruption strains.

in CC-125: CC-5427cop11-B9-1 [PH133], CC-5428cop11-B9-2 [PH134]

 

aCRY in SAG 73.72

Animal-like cryptochrome (aCRY) disruption strains in SAG 73.72.

Clones: CC-5396 ∆acry-A3 [PH73], CC-5397acry-E10 [PH74]

 

UVR8

UV RESISTANCE LOCUS8 (UVR8) disruption strains.

in CC-3403: CC-5439uvr8-I7-E12 [PH048], CC-5440uvr8-I8-E4 [PH050]

in CC-125: CC-5441uvr8-A2 [PH125], CC-5442uvr8-F3 [PH126]

 

PHOT

Phototropin (PHOT) disruption strains.

in CC-125:  CC-5391phot-C41 [PH13], CC-5392phot-B5 [PH15]

in SAG11-32b: CC-5429phot-A5 [PH135], CC-5430phot-A7 [PH136] 

 

PHOT/∆UVR8

Phototropin (PHOT) and UVR8 double knock-out strains.

in CC-3403:  CC-5444uvr8-∆phot-D5 [PH067], CC-5445uvr8-∆phot-F6 [PH068]

in CC-125: CC-5446 ∆photuvr8-B3 [PH130], CC-5447 ∆photuvr8-H9 [PH131] 

 

Other Knock-out strains

 

VGCC

Voltage-gated calcium channel (VGCC) disruption strain.

in CC-3403:CC-5435vgcc-F3 [PH038] , CC-5436vgcc-H3 [PH039]
in CC-125: CC-5433vgcc-B9 [PH063], CC-5434vgcc-C9 [PH064]

 

MAT3

MAT3 disruption strains:

in CC-125: CC-5394 ∆mat3-A5 [PH57], CC-5395mat3-E8 [PH58]

 

PSR1

PSR1 disruption strain.

in CC-3403: CC-5424psr1-D10 [PH011]

 

2-LOG

in CC-125: CC-5450 ∆2-LOG-C9 mt+ [PH147], CC-5449 ∆2-LOG-A12 mt+ [PH146]

 

CiliK (GCLK1)

in SAG 11-32b: CC-5542 ∆CiliK-D2 (GCLK1) mt+ [PH171], CC-5543 ∆CiliK-D1 (GCLK1) mt+ [PH170]

 

4-IPT

in CC-125: CC-5540 ∆4-IPT-F6 mt+ [PH219], CC-5541 ∆4-IPT-A10 mt+ [PH220]

 

CAV2

in CC-125: CC-5544 ∆CAV2-B12 mt+ [PH168], CC-5545 ∆CAV2-A11 mt+ [PH169]

 

 

Here is a list of previous strains sent to the Chlamydomonas Ressource Center.