Strains deposited by the Hegemann lab at the Chlamydomonas Resource Center
Visit www.chlamy.de for more info or contact CRISPR@chlamy.de. Please verify your strains by sequencing of the target region.
Photoreceptor Knock-outs
∆COP1/2
Chlamyopsin (COP1/2) disruption strains.
in CC-125: CC-5398 ∆cop1/2-F4 [PH77], CC-5399 ∆cop1/2-F7 [PH78]
∆ChR1 (COP3)
Channelrhodopsin 1 (ChR1) disruption strains:
in CC-3403:CC-5546 ∆chr1-M6-F4-3 [PH167], CC-5547 ∆chR1-E12 [PH166]
in SAG 11-32b: CC-5550 ∆chR1-H6 mt+ [PH160], CC-5551 ∆chR1-F1 mt+ [PH163]
∆ChR2 (COP4)
Channelrhodopsin 2 (ChR2) disruption strains:
in CC-3403: CC-5548 ∆chR2-F12 mt- [PH162], CC-5549 ∆chR2-D11 mt- [PH161],
in SAG 11-32b: CC-5552 ∆chR2-H9 mt+ [PH165], NA ∆chR2-H9 mt+ [PH164]
∆ChR1 ∆ChR2
Channelrhodopsin 1+2 double Knock-out strains:
in CC-125: CC-5499 ∆ChR1 ∆ChR2 [PH128],
∆COP5 (HKR1)
Chlamyopsin 5 (COP5, HKR1) disruption strains.
in CC-3403: CC-5437 ∆cop5.20-B2 [PH042], CC-5438 ∆cop5.12-H3 [PH043]
in CC-125: CC-5443 ∆cop5-D11 [PH065], CC-5448 ∆cop5-E6 [PH103]
∆COP6 (HKR2)
Chlamyopsin 6 (COP6, HKR2) disruption strains.
in CC-125: CC-5431 ∆cop6-F9 [PH143], CC-5432 ∆cop6-G4 [PH142]
∆COP8 (HKR4)
Chlamyopsin 8 (COP8, HKR4) disruption strains.
in CC-3403: CC-5425 ∆cop8-E9D1 [PH101], CC-5426 ∆cop8-F1A1 [PH102]
∆COP11/12 (HKR7/8)
Chlamyopsin 11/12 (COP11/12, HKR7/8) disruption strains.
in CC-125: CC-5427 ∆cop11-B9-1 [PH133], CC-5428 ∆cop11-B9-2 [PH134]
∆aCRY in SAG 73.72
Animal-like cryptochrome (aCRY) disruption strains in SAG 73.72.
Clones: CC-5396 ∆acry-A3 [PH73], CC-5397 ∆acry-E10 [PH74]
∆UVR8
UV RESISTANCE LOCUS8 (UVR8) disruption strains.
in CC-3403: CC-5439 ∆uvr8-I7-E12 [PH048], CC-5440 ∆uvr8-I8-E4 [PH050]
in CC-125: CC-5441 ∆uvr8-A2 [PH125], CC-5442 ∆uvr8-F3 [PH126]
∆PHOT
Phototropin (PHOT) disruption strains.
in CC-125: CC-5391 ∆phot-C41 [PH13], CC-5392 ∆phot-B5 [PH15]
in SAG11-32b: CC-5429 ∆phot-A5 [PH135], CC-5430 ∆phot-A7 [PH136]
∆PHOT/∆UVR8
Phototropin (PHOT) and UVR8 double knock-out strains.
in CC-3403: CC-5444 ∆uvr8-∆phot-D5 [PH067], CC-5445 ∆uvr8-∆phot-F6 [PH068]
in CC-125: CC-5446 ∆phot ∆uvr8-B3 [PH130], CC-5447 ∆phot ∆uvr8-H9 [PH131]
Other Knock-out strains
∆VGCC
Voltage-gated calcium channel (VGCC) disruption strain.
in CC-3403:CC-5435 ∆vgcc-F3 [PH038] , CC-5436 ∆vgcc-H3 [PH039]
in CC-125: CC-5433 ∆vgcc-B9 [PH063], CC-5434 ∆vgcc-C9 [PH064]
∆MAT3
MAT3 disruption strains:
in CC-125: CC-5394 ∆mat3-A5 [PH57], CC-5395 ∆mat3-E8 [PH58]
∆PSR1
PSR1 disruption strain.
in CC-3403: CC-5424 ∆psr1-D10 [PH011]
∆2-LOG
in CC-125: CC-5450 ∆2-LOG-C9 mt+ [PH147], CC-5449 ∆2-LOG-A12 mt+ [PH146]
∆CiliK (GCLK1)
in SAG 11-32b: CC-5542 ∆CiliK-D2 (GCLK1) mt+ [PH171], CC-5543 ∆CiliK-D1 (GCLK1) mt+ [PH170]
∆4-IPT
in CC-125: CC-5540 ∆4-IPT-F6 mt+ [PH219], CC-5541 ∆4-IPT-A10 mt+ [PH220]
∆CAV2
in CC-125: CC-5544 ∆CAV2-B12 mt+ [PH168], CC-5545 ∆CAV2-A11 mt+ [PH169]
Here is a list of previous strains sent to the Chlamydomonas Ressource Center.