Humboldt-Universität zu Berlin - Institut für Biologie


Prof. Dr. Athina Zouni




Adrian Bothe, Athina Zouni and Frank Müh, Refined definition of the critical micelle concentration and application to alkyl maltosides used in membrane protein research RSC Adv., 2023,13, 9387-9401


Sascha Morlock, Senthil K. Subramanian, Athina Zouni and Fred Lisdat, Closing the green gap of photosystem I with synthetic fluorophores for enhanced photocurrent generation in photobiocathodes Chem. Sci., 2023, Advance Article




Philipp S. Simon, Hiroki Makita, Isabel Bogacz, Franklin Fuller, Asmit Bhowmick, Rana Hussein, Mohamed Ibrahim, Miao Zhang, Ruchira Chatterjee, Mun Hon Cheah, Petko Chernev, Margaret D. Doyle, Aaron S. Brewster, Roberto Alonso-Mori, Nicholas K. Sauter, Uwe Bergmann, Holger Dobbek, Athina Zouni, Johannes Messinger, Jan Kern, Vittal K. Yachandra and Junko Yano, Capturing the sequence of events during the wateroxidation reaction in photosynthesis using XFELs FEBS Lett. (2022)


Sascha Morlock*, Senthil Kumar Subramanian, Athina Zouni, Fred Lisdat**, Bio-inorganic hybrid structures for direct electron transfer to photosystem I in photobioelectrodes Biosens. Bioelectron. 214 (2022), 11449


Golub, J. Gätcke, S. Subramanian, A. Kölsch, T. Darwish, J. K. Howard, A. Feoktystov, O. Matsarskaia, A. Martel, L. Porcar, A. Zouni, and J. Pieper*, “Invisible” Detergents Enable a Reliable Determination of Solution Structures of Native Photosystems by Small-Angle Neutron Scattering J. Phys. Chem. B 2022, 126, 15, 2824–2833




Thomas Fransson, Roberto Alonso-Mori, Ruchira Chatterjee, Mun Hon Cheah, Mohamed Ibrahim, Rana Hussein, Miao Zhang, Franklin Fuller, Sheraz Gul, In-Sik Kim, Philipp S. Simon, Isabel Bogacz, Hiroki Makita, Casper de Lichtenberg, Sanghoon Song, Alexander Batyuk, Dimosthenis Sokaras, Ramzi Massad, Margaret Doyle, Alexander Britz, Clemens Weninger, Athina Zouni, Johannes Messinger, Vittal K. Yachandra, Junko Yano, Jan Kern, and Uwe Bergmann, Effects of x-ray free-electron laser pulse intensity on the Mn Kβ1,3 x-ray emission spectrum in photosystem II—A case study for metalloprotein crystals and solutions Structural Dynamics 8, 064302 (2021)


Rana Hussein, Mohamed Ibrahim, Asmit Bhowmick, Philipp S. Simon, Ruchira Chatterjee, Louise Lassalle, Margaret Doyle, Isabel Bogacz, In-Sik Kim, Mun Hon Cheah, Sheraz Gul, Casper de Lichtenberg, Petko Chernev, Cindy C. Pham, Iris D. Young, Sergio Carbajo, Franklin D. Fuller, Roberto Alonso-Mori, Alex Batyuk, Kyle D. Sutherlin, Aaron S. Brewster, Robert Bolotovsky, Derek Mendez, James M. Holton, Nigel W. Moriarty, Paul D. Adams, Uwe Bergmann, Nicholas K. Sauter, Holger Dobbek, Johannes Messinger, Athina Zouni, Jan Kern, Vittal K. Yachandra & Junko Yano, Hussein, R., Ibrahim, M., Bhowmick, A. et al., Structural dynamics in the water and proton channels of photosystem II during the S2 to S3 transition. Nat Commun 12, 6531 (2021)


Stephen M. Keable, Adrian Kölsch, Philipp S. Simon, Medhanjali Dasgupta, Ruchira Chatterjee, Senthil Kumar Subramanian, Rana Hussein, Mohamed Ibrahim, In-Sik Kim, Isabel Bogacz, Hiroki Makita, Cindy C. Pham, Franklin D. Fuller, Sheraz Gul, Daniel Paley, Louise Lassalle, Kyle D. Sutherlin, Asmit Bhowmick, Nigel W. Moriarty, Iris D. Young, Johannes P. Blaschke, Casper de Lichtenberg, Petko Chernev, Mun Hon Cheah, Sehan Park, Gisu Park, Jangwoo Kim, Sang Jae Lee, Jaehyun Park, Kensuke Tono, Shigeki Owada, Mark S. Hunter, Alexander Batyuk, Roland Oggenfuss, Mathias Sander, Serhane Zerdane, Dmitry Ozerov, Karol Nass, Henrik Lemke, Roman Mankowsky, Aaron S. Brewster, Johannes Messinger, Nicholas K. Sauter, Vittal K. Yachandra, Junko Yano, Athina Zouni & Jan Kern, Keable, S.M., Kölsch, A., Simon, P.S. et al., Room temperature XFEL crystallography reveals asymmetry in the vicinity of the two phylloquinones in photosystem I. Sci Rep 11, 21787 (2021)


Nikoloz Nioradze, Dmitri Ciornii, Adrian Kölsch, Gero Göbel, Dimitri E. Khoshtariya, Athina Zouni, Fred Lisdat, Electrospinning for building 3D structured photoactive biohybrid electrodes Bioelectrochemistry 142 (2021) 107945


Mohamed Ibrahim, Nigel W. Moriarty, Jan Kern, James M. Holton, Aaron S. Brewster, Asmit Bhowmick, Uwe Bergmann, Athina Zouni, Johannes Messinger, Vittal K. Yachandra, Junko Yano, Holger Dobbek, Nicholas K. Sauter, and Paul D. Adams, Reply to Wang et al.: Clear evidence of binding of Ox to the oxygen-evolving complex of photosystem II is best observed in the omit map, Proc. Natl. Acad. Sci. U. S. A. 2021, 118 (24)


Sascha Morlock,* Senthil K. Subramanian, Athina Zouni, and Fred Lisdat, Scalable Three-Dimensional Photobioelectrodes Made of Reduced Graphene Oxide Combined with Photosystem I, ACS Appl. Mater. Interfaces 2021, 13, 9, 11237–11246


Maksym Golub, Adrian Kölsch, Artem Feoktystov, Prof. Dr. Athina Zouni and Jörg Pieper, Insights into Solution Structures of Photosynthetic Protein Complexes from Small-Angle Scattering Methods, Crystals 2021, 11(2), 203




Maksym Golub, Rana Hussein, Mohamed Ibrahim, Max Hecht, Dietmar Christian, Florian Wieland, Anne Martel, Barbara Machado, Athina Zouni, and Jörg Pieper, Solution Structure of the Detergent-Photosystem II Core Complex Investigated by Small Angle Scattering Techniques, J. Phys. Chem. B 2020, 124, 39, 8583–8592


Kölsch, A., Radon, C., Golub, M., Baumert, A., Bürger, J., Mielke, T., Lisdat,F., Feoktystov, A., Pieper, J., Zouni, A., Wendler, P., Current limits of structural biology: the transientinteraction between cytochrome c6 and photosystem I, Current Research in Structural Biology 2 (2020) 171–179


Ibrahim, M.; Fransson, T.; Chatterjee, R.; Cheah, M. H.; Hussein, R.; Lassalle, L.; Sutherlin, K. D.; Young, I. D.; Fuller, F. D.; Gul, S.; Kim, I.-S.; Simon, P. S.; de Lichtenberg, C.; Chernev, P.; Bogacz, I.; Pham, C. C.; Orville, A. M.; Saichek, N.; Northen, T.; Batyuk, A.; Carbajo, S.; Alonso-Mori, R.; Tono, K.; Owada, S.; Bhowmick, A.; Bolotovsky, R.; Mendez, D.; Moriarty, N. W.; Holton, J. M.; Dobbek, H.; Brewster, A. S.; Adams, P. D.; Sauter, N. K.; Bergmann, U.; Zouni, A.; Messinger, J.; Kern, J.; Yachandra, V. K.; Yano, J., Untangling the sequence of events during the S2 → S3 transition in photosystem II and implications for the water oxidation mechanism. Proc. Natl. Acad. Sci. U. S. A. 2020, 117 (23), 12624-12635


Müh, F.; Zouni, A., Structural basis of light‐harvesting in the photosystem II core complex. Protein Science 2020, 29 (5), 1090-1119


Gerland, L.; Friedrich, D.; Hopf, L.; Donovan, E. J.; Wallmann, A.; Erdmann, N.; Diehl, A.; Bommer, M.; Buzar, K.; Ibrahim, M.; Schmieder, P.; Dobbek, H.; Zouni, A.; Bondar, A.-N.; Dau, H.; Oschkinat, H., pH‐Dependent Protonation of Surface Carboxylate Groups in PsbO Enables Local Buffering and Triggers Structural Changes. ChemBioChem 2020,21, 1597 – 1604




Cheah, M. H.; Zhang, M.; Shevela, D.; Mamedov, F.; Zouni, A.; Messinger, J., Assessment of the manganese cluster’s oxidation state via photoactivation of photosystem II microcrystals. Proc. Natl. Acad. Sci. U. S. A. 2019, 201915879


Kemmler, L.; Ibrahim, M.; Dobbek, H.; Zouni, A.; Bondar, A.-N., Dynamic water bridging and proton transfer at a surface carboxylate cluster of photosystem II. Phys. Chem. Chem. Phys., 2019,21, 25449-25466


Sidabras, J. W.; Duan, J.; Winkler, M.; Happe, T.; Hussein, R.; Zouni, A.; Suter, D.; Schnegg, A.; Lubitz, W.; Reijerse, E. J., Extending electron paramagnetic resonance to nanoliter volume protein single crystals using a self-resonant microhelix. Sci. Adv. 2019; 5: eaay1394


Kern, J.; Müh, F.; Zouni, A., Structural studies on tetrapyrrole containing proteins enabled by femtosecond X-ray pulses. Metabolism, Structure and Function of Plant Tetrapyrroles: Control Mechanisms of Chlorophyll Biosynthesis and Analysis of Chlorophyll-Binding Proteins 2019, 33


Ruocheng Han, Katharina Rempfer, Miao Zhang, Prof. Dr. Holger Dobbek, Prof. Dr. Athina Zouni, Prof. Dr. Holger Dau, Prof. Dr. Sandra Luber, Investigating the Structure and Dynamics of Apo‑Photosystem II. ChemCatChem 2019,11, 4072–4080


Chatterjee R, Lasalle L, Gul S, Fuller FD, Young ID, Ibrahim M, de Lichtenberg C, Cheah MH, Zouni A, Messinger J, Yachandra VK, Kern J, Yano J Structural isomers of the S2 state in photosystem II: do they exist at room temperature and are they important for function?. Physiol. Plant. 166, 2019


Ciornii D, Kölsch A, Zouni A, Lisdat F., Exploiting new ways for a more efficient orientation and wiring of PSI to electrodes: A fullerene C70 approach. Electrochim. Acta Volume 299, 10 March 2019, Pages 531-539




Riedel M, Wersig J, Ruff A, Schuhmann W, Zouni A, Lisdat F., A Z-Scheme-Inspired Photobioelectrochemical H2O/O2 Cell with a 1 V Open-Circuit Voltage Combining Photosystem II and PbS Quantum Dots. Angew.Chem.Int.Ed. 2019,58,801 –805


Jan Kern, Ruchira Chatterjee, Iris D. Young, Franklin D. Fuller, Louise Lassalle, Mohamed Ibrahim, Sheraz Gul, Thomas Fransson, Aaron S. Brewster, Roberto Alonso-Mori, Rana Hussein, Miao Zhang, Lacey Douthit, Casper de Lichtenberg, Mun Hon Cheah, Dmitry Shevela, Julia Wersig, Ina Seuffert, Dimosthenis Sokaras, Ernest Pastor, Clemens Weninger, Thomas Kroll, Raymond G. Sierra, Pierre Aller, Agata Butryn, Allen M. Orville, Mengning Liang, Alexander Batyuk, Jason E. Koglin, Sergio Carbajo, Sébastien Boutet, Nigel W. Moriarty, James M. Holton, Holger Dobbek, Paul D. Adams, Uwe Bergmann, Nicholas K. Sauter, Athina Zouni, Johannes Messinger, Junko Yano & Vittal K. Yachandra, Structures of the intermediates of Kok’s photosynthetic water oxidation clock. Nature 563, 421–425 (2018)


Feifel SC, Stieger KR, Hejazi M, Wang X, Ilbert M, Zouni A, Lojou E, Lisat F., Dihemic c4 -type cytochrome acting as a surrogate electron conduit: Artificially interconnecting a photosystem I supercomplex with electrodes. Electrochemistry Communications 91, 2018 Pages 49-53.


Kölsch A, Hejazi M, Stieger KR, Feifel SC, Kern JF, Müh F, Lisdat F, Lokstein H, Zouni A., Insights into the binding behavior of native and non-native cytochromes to Photosystem I from Thermosynechococcus elongatus. J. Biol. Chem. (2018) 293(23) 9090 –9100


Stieger K, Feifel SC, Lokstein H, Hejazi M, Zouni A, Lisdat., Biohybride Architekturen für eine effiziente Umwandlung von Licht in elektrische Energie durch Integration von Photosystem I in skalierbare mesoporöse 3D Elektroden. Wiss. Beitr. TH Wildau 22: 17-24. Mar 2018.




Yachandra V, Kern J, Zouni A, Messinger J, Bergmann U, Alonso-Mori R, Wernet P, Sauter N, Yano J., Taking snapshots of photosynthetic water oxidation with an X-ray laser. Acta Crystallographica Section A Foundations and Advances, 73



Lassalle, Young ID, Ibrahim M, Chatterjee R, Gul S, Fuller F, Brewster A, Douthit L, Pastor E, Sauter N, Zouni A, Kern J, Yachandra V and Yano J., Exploring the dynamic of PSII at room temperature by simultaneous femtosecond X-ray spectroscopy and diffraction. Acta Crystallographica Section A Foundations and Advances, 73, a114



Ciornii D, Riedel M, Stieger KR, Feifel SC, Hejazi M, Lokstein H, Zouni A, Lisdat F., Bioelectronic Circuit on a 3D Electrode Architecture: Enzymatic Catalysis Interconnected with Photosystem I. J. Am. Chem. Soc. 2017, 139, 16478−16481


Hussein R, Mohamed Ibrahim M, Chatterjee R, Coates L, Müh F, Vittal K. Yachandra V.K., Yano J, Jan Kern J, Dobbek H, and Zouni A., Optimizing Crystal Size of Photosystem II by Macroseeding: Toward Neutron Protein Crystallography. Cryst. Growth Des. 2018, 18, 85−94


Bommer M, Coates L, Dau H, Zouni A, Dobbek H., Protein crystallization and initial neutron diffraction studies of the photosystem II subunit PsbO. Acta Cryst. (2017). F73, 525-531


Zhang M, Bommer M, Chatterjee R, Hussein R, Yano J, Dau H, Kern J, Dobbek H, Zouni A., Structural insights into the light-driven auto-assembly process of the water-oxidizing Mn4CaO5-cluster in photosystem II. eLife 2017;6:e26933


Skandary S, Müh F, Ashraf I, Ibrahim M, Metzger M, Zouni A, Meixner AJ, and Brecht M., Role of missing carotenoid in reducing the fluorescence of single monomeric photosystem II core complexes. Phys. Chem. Chem. Phys., 2017,19, 13189-13194


Dmitri Ciornii, Feifel SC, Hejazi M, Kölsch A, Lokstein H, Zouni A, and Lisdat F., Construction of photobiocathodes using multi-walled carbon nanotubes and photosystem I. PSS. Phys. Status Solidi A 214, No. 9 (2017)


Golub M, Hejazi M, Kölsch A, Lokstein H, Wieland D.C.F, Zouni A, and Pieper J., Solution structure of monomeric and trimeric photosystem I of Thermosynechococcus elongatus investigated by small-angle X-ray scattering. Photosynth Res (2017) 133:163–173


Franklin D Fuller, Sheraz Gul, Ruchira Chatterjee, E Sethe Burgie, Iris D Young, Hugo Lebrette, Vivek Srinivas, Aaron S Brewster, Tara Michels-Clark, Jonathan A Clinger, Babak Andi, Mohamed Ibrahim, Ernest Pastor, Casper de Lichtenberg, Rana Hussein, Christopher J Pollock, Miao Zhang, Claudiu A Stan, Thomas Kroll, Thomas Fransson, Clemens Weninger, Markus Kubin, Pierre Aller, Louise Lassalle, Philipp Bräuer, Mitchell D Miller, Muhamed Amin, Sergey Koroidov, Christian G Roessler, Marc Allaire, Raymond G Sierra, Peter T Docker, James M Glownia, Silke Nelson, Jason E Koglin, Diling Zhu, Matthieu Chollet, Sanghoon Song, Henrik Lemke, Mengning Liang, Dimosthenis Sokaras, Roberto Alonso-Mori, Athina Zouni, Johannes Messinger, Uwe Bergmann, Amie K Boal, J Martin Bollinger Jr, Carsten Krebs, Martin Högbom, George N Phillips Jr, Richard D Vierstra, Nicholas K Sauter, Allen M Orville, Jan Kern, Vittal K Yachandra & Junko Yano., Drop-on-demand sample delivery for studying biocatalysts in action at X-ray free-electron lasers. Nature Methods VOL.14 NO.4 APRIL 2017




Iris d. Young1, Mohamed Ibrahim, Ruchira Chatterjee, Sheraz Gul, Franklin d. Fuller, Sergey Koroidov, Aaron S. Brewster, Rosalie Tran, Roberto Alonso-Mori, Thomas Kroll, Tara Michels-Clark, Hartawan Laksmono, Raymond G. Sierra, Claudiu A. Stan, Rana Hussein, Miao Zhang, Lacey Douthit, Markus Kubin, Casper de Lichtenberg, Long Vo Pham, Håkan Nilsson, Mun Hon Cheah, Dmitriy Shevela, Claudio Saracini, Mackenzie A. Bean, Ina Seuffert, Dimosthenis Sokaras, Tsu-Chien Weng, Ernest Pastor, Clemens Weninger, Thomas Fransson, Louise Lassalle, Philipp Bräuer, Pierre Aller, Peter T. Docker, Babak Andi, Allen M. Orville, James M. Glownia, Silke Nelson, Marcin Sikorski, Diling Zhu, Mark S. Hunter, Thomas J. Lane, Andy Aquila, Jason E. Koglin, Joseph Robinson, Mengning Liang, Sébastien Boutet, Artem Y. Lyubimov, Monarin Uervirojnangkoorn, Nigel W. Moriarty, Dorothee Liebschner, Pavel V. Afonine, David G. Waterman, Gwyndaf Evans, Philippe Wernet, Holger Dobbek, William I. Weis, Axel T. Brunger, Petrus H. Zwart, Paul D. Adams, Athina Zouni, Johannes Messinger, Uwe Bergmann, Nicholas K. Sauter, Jan Kern, Vittal K. Yachandra & Junko Yano, Structure of photosystem II and substrate binding at room temperature. Nature 540, 453–457 (2016)


Stieger KR, Feifel SC, Lokstein H, Hejazi M, Zouni A, Lisdat F., Biohybrid architectures for efficient light-to-current conversion based on photosystem I within scalable 3D mesoporous electrodes. J. Mater. Chem. A, 2016, 4, 17009-17017


Alonso-Mori R, Asa K, Bergmann U, Brewster AS, Chatterjee R, Cooper JK, Frei HM, Fuller FD, Goggins E, Gul S, Fukuzawa H, Iablonskyi D, Ibrahim M, Katayama T, Kroll T, Kumagai Y, McClure BA, Messinger J, Motomura K, Nagaya K, Nishiyama T, Saracini C, Sato Y, Sauter NK, Sokaras D, Takanashi T, Togashi T, Ueda K, Weare WW, Weng TC, Yabashi M, Yachandra VK, Young ID, Zouni A, Kern JF, Yano J., Towards characterization of photo-excited electron transfer and catalysis in natural and artificial systems using XFELs. Faraday Discuss. 2016 ;194:621-638


Bommer M, Bondar AN, Zouni A, Dobbek H, Dau H., Crystallographic and Computational Analysis of the Barrel Part of the PsbO Protein of Photosystem II: Carboxylate–Water Clusters as Putative Proton Transfer Relays and Structural Switches. Biochemistry, 2016, 55 (33), 4626–4635


Sauter NK, Echols N, Adams PD, Zwart PH, Kern J, Brewster AS, Koroidov S, Alonso-Mori R, Zouni A, Messinger J, Bergmann U, Yano J, Yachandra V, No observable conformational changes in PSII. Nature 533, E1–E2 (2016)


Stieger KR, Ciornii D, Kölsch A, Hejazi M, Lokstein H, Feifel SC, Zouni A, Lisdat F., Engineering of supramolecular photoactive protein architectures: the defined co-assembly of photosystem I and cytochrome c using a nanoscaled DNA-matrix. Nanoscale, 2016, 8, 10695-10705


Roessler CG, Agarwal R, Allaire M, Alonso-Mori R, Andi B, Bachega JF, Bommer M, Brewster AS, Browne MC, Chatterjee R, Cho E, Cohen AE, Cowan M, Datwani S, Davidson VL, Defever J, Eaton B, Ellson R, Feng Y, Ghislain LP, Glownia JM, Han G, Hattne J, Hellmich J, Héroux A, Ibrahim M, Kern J, Kuczewski A, Lemke HT, Liu P, Majlof L, McClintock WM, Myers S, Nelsen S, Olechno J, Orville AM, Sauter NK, Soares AS, Soltis SM, Song H, Stearns RG, Tran R, Tsai Y, Uervirojnangkoorn M, Wilmot CM, Yachandra V, Yano J, Yukl ET, Zhu D, Zouni A., Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography. Structure, 2016, 24(4)


Baxter E.L, Aquila L, Alonso-Mori R, Barnes C.O, Bonagura C.A, Brehmer W, Brunger A.T, Calero G, Caradoc-Davies T.T, Chatterjee R, Degrado W.F, Fraser J.S, Ibrahim M, Kern J, Kobilka B.K, Kruse A.C, Larsson K.M, Lemke, H.T, Lyubimov A.Y, Manglik A, McPhillips S.E, Norgren E, Pang S.S, Soltis S.M, Song J, Thomaston J, Tsai Y, Weis W.I, Woldeyes R.A, Yachandra V, Yano J, Zouni A, and Cohen A.E., High-density grids for efficient data collection from multiple crystals. Acta Crystallogr D Struct Biol. 2016 Jan;72(Pt 1):2-11


Sierra R.G, Gati C, Laksmono H, Han Dao E, Gul S, Fuller F, Kern J, Chatterjee R, Ibrahim M, Brewster A.S, Young I.D, Michels-Clark T, Aquila A, Liang M, Hunter M.S, Koglin J.E, Boutet S, Junco E.A, Hayes B, Bogan M.J, Hampton C.Y, Puglisi E.V, Sauter N.K, Stan C.A, Zouni A, Yano J, Yachandra V.K, Soltis S.M, Puglisi J.D, and DeMirci H., Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II. Nat Methods 13, 59–62 (2016)




Ibrahim M, Chatterjee R, Hellmich J, Tran R, Bommer M, Yachandra V.K, Yano J, Kern J and Zouni A., Improvements in serial femtosecond crystallography of photosystem II by optimizing crystal uniformity using microseeding procedures. Struct Dyn. 2015;2(4):041705


Müh F, DiFiore D, and Zouni A., Influence of poly(ethylene glycol) on the micelle formation of alkyl maltosides used in membrane protein crystallization. Phys. Chem. Chem. Phys., 2015, 17, 11678


Skandary S, Hussels M, Konrad A, Renger T, Müh F, Bommer M, Zouni A, Meixner A.J, and Brecht M., Variation of Exciton-Vibrational Coupling in Photosystem II Core Complexes from Thermosynechococcus elongatus As Revealed by Single-Molecule Spectroscopy. J. Phys. Chem. B 2015, 119, 4203−4210


Müh F., Zouni A. (2015). Cytochrome b559 in photosystem II. In: Cramer WA, Kallas T (eds) Cytochrome Complexes: Evolution, Structures, Energy Transduction, and Signaling. Springer, Dordrecht




Kern, J., Tran, R., Alonso-Mori, R., Koroidov, S., Echols, N., Hattne, J., Ibrahim M., Gul, S, Laksmono, H., Sierra, R. G., Gildea, R. J., Han, G., Hellmich, J., Lassalle-Kaiser, B., Chatterjee, R., Brewster, A. S., Stan, C. A., Glöckner, C., Lampe, A., DiFiore, D., Milathianaki, D., Fry, A. R., Seibert, M. M., Koglin, J. E., Gallo, E., Uhlig, J., Sokaras, D., Weng, T.-C., Zwart, P. H., Skinner, D. E., Bogan, M. J., Messerschmidt, M., Glatzel, P., Williams, G. J., Boutet, S., Adams, P. D., Zouni, A., Messinger, J., Sauter, N. K., Bergmann, U., Yano, J., and Yachandra, V. K., Taking snapshots of photosynthetic water oxidation using femtosecond X-ray diffraction and spectroscopy. Nat Commun 5, 4371 (2014)


Hellmich J, Bommer M, Burkhardt A, Ibrahim M, Kern J, Meents A, Müh F, Dobbek H, Zouni A., Native-like Photosystem II Superstructure at 2.44 Å Resolution through Detergent Extraction from the Protein Crystal. Structure 2014, 22, 1607-1615


Tran R., Kern J., Hattne J., Kroidov S., Hellmich J., Alonso-Mori R., Sauter N.K., Bergmann U., Messinger J., Zouni A., Yano J., Yachandra V.K., The Mn4Ca photosynthetic water-oxidation catalyst studied by simultaneous X-ray spectroscopy and crystallography using an X-ray free-electron laser. Phil. Trans. R. Soc. B (2014) 369 20130324


Brecht M, Skandary S., Hellmich J., Glöckner C., Konrad A., Hussels M., Meixner A. J., Zouni A., Schlodder E., Spectroscopic properties of photosystem II core complexes from Thermosynechococcus elongatus revealed by single-molecule experiments. Biochimica et Biophysica Acta 1837 (2014) 773–781


Hattne J, Ecols N., Tran R., Kern J., Gildea R. J., Zouni A., Yano J., Bergmann U., Yachandra V. K., Adams P. D., Sater N. K., Accurate macromolecular structures using minimal measurements from X-ray free-electron lasers, Nat Methods 11, 545–548 (2014)




Glöckner C, Kern J, Broser M, Zouni A, Yachandra V, Junko Y., Structural Changes of the Oxygen-evolving Complex in Photosystem II during the Catalytic Cycle. J. Biol. Chem. 2013, 288, 22607-22620


Kern J, Alonso-Mori R, Tran R, Hattne J, Gildea R.G., Echols N, Glöckner C, Hellmich J, Laksmono H, Sierra R G, Lassalle-Kaiser B, Koroidov S, Lampe A, Han G, Gul S, DiFiore D, Milathianaki D, Fry A R, Miahnari A, Schafer D W, Messerschmidt M, Seibert M M, Jason Koglin E, Sokaras D, Weng T C, Sellberg J, Latimer M J, Grosse-Kunstleve R W, Zwart P H, White W H, Glatzel P, Adams P D, Bogan M J, Williams G j, Boutet S, Messinger J, Zouni A, Sauter N K, Yachandra V K, Bergmann U, Yano J., Simultaneous Femtosecond X-Ray Spectroscopy and Diffraction of Photosystem II at Room Temperature. Science 2013, 340, 491-495


Müh F., Zouni A., The nonheme iron in photosystem II, Photosynth Res (2013), 116, 295-314


Brose K., Zouni A., Müh F., Mroginski M.A., Maultzsch J., Simulations of the polarization-dependent Raman intensity of β-carotene in photosystem II crystals. Chemical Physics 2013, 418, 65-73




Kern, R. Alonso-Mori, J. Hellmich, R. Tran, J. Hattne, H. Laksmono, C. Glöckner, N. Echols, R.G. Sierra, J. Sellberg, B. Lassalle-Kaiser, R. J. Gildea, P. Glatzel, R. W. Grosse-Kunstleve, M. J. Latimer, T. A. McQueen, D. DiFiore, A. R. Fry, M. Messerschmidt, A. Miahnahri, D. W. Schafer, M. M., Seibert, D. Sokaras, T. C. Weng, P. H. Zwart, W. E. White, P. D. Adams, M. J. Bogan, S. Boutet, G. J. Williams, J. Messinger, N. K. Sauter, A. Zouni, U. Bergmann, J. Yano, V. K. Yachandra, Room Temperature Femtosecond X-ray Diffraction of Photosystem II Microcrystals, Proc. Natl. Acad. Sci. U. S. A. 2012, 109, 9721-9726


Burkhardt A., Warmer M., Pannneerselvam S., Wagner, A., Zouni A., Glöckner C., Reimer R., Hohenberg H, Meents A., Fast high-pressure freezing of protein crystals in their mother liquor, Acta Cryst 2012, F68, 495-500


Müh F., Glöckner C., Hellmich J., Zouni A., Light-induced quinone reduction in photosystem II. Biochim Biophys Acta, 2012, 1817, 44-65


Alonso-Mori R., Kern J., Gildea R. J., Sokaras D., Wenig T.-C., Lassalle-Kaiser B., Tran R., Hattne J., Laksmono H., Hellmich J., Glöckner C., Echols N., Sierra R. G., Schafer D. W., Sellberg J., Kenney C., Herbst R., Pines J., Hart P., Herrmann S., Grosse-Kunstleve R. W., Latimer M. J., Fry A. R., Messerschmidt M. M., Miahnahri A., Seibert M. M., Zwart P. H., White W. E., Adams P. D., Bogan M. J., Boutet S., Williams G. J., Zouni A., Messinger J., Glatzel P., Sauter N. K., Yachandra V. K., Yano J., Bergmann U.. Energy-dispersive X-ray emission spectroscopy using an X-ray free-electron laser in a shot-by-shot mode, Proc. Natl. Acad. Sci. U. S. A. 2012, 109, 19103-19107


Sierra R. G., Laksmono H, Kern,J., Tran R., Hattne J., Alonso-Mori,R., Lassalle-Kaiser B., Glöckner C., Hellmich J., Schafer D. W., Echols N., Gildea R. J., ,b Grosse-Kunstleve R. W., Sellberg J., McQueen T. A., Fry A. R., Messerschmidt M. M., Miahnahri A., Seibert M. M., Hampton C. Y., Starodub D., Loh N. D., Sokaras D., Wenig T. C., Zwart P.H., Glatzel P, Milathianaki D., White W. E., Adams P. D., Williams G. J., Boutet S., Zouni A., Messinger J., Sauter N. K., Bergmann U., Yano J., Yachandra V. K., Bogan M., Nanoflow electrospinning serial femtosecond crystallography, Acta Cryst. (2012). D68, 1584-1587




Broser M, Glöckner C, Gabdulkhakov A, Guskov A, Buchta J, Kern J, Müh F, Dau H, Saenger W, Zouni A., Structural Basis of Cyanobacterial Photosystem II Inhibition by the Herbicide Terbutryn, J. Biol. Chem. 2011, 286, 15964-15972


Müh, F., Zouni, A., Light-induced water oxidation in photosystem II, Front. Biosci. (Landmark Ed) 2011, 16(8), 3072–3132




Broser, M., Gabdulkhakov, A., Kern, J., Guskov, A., Müh, F., Saenger, W., Zouni, A., Crystal Structure of Monomeric Photosystem II from Thermosynechococcus elongatus at 3.6 Å Resolution. J. Biol. Chem. 2010, 285, 26255-26262.


Guskov, A., Gabdulkhakov, A., Broser, M., Glöckner, C., Hellmich, J., Kern, J., Frank, J., Müh, F., Saenger, W., Zouni, A., Recent Progress in the Crystallographic Studies of Photosystem II. ChemPhysChem 2010, 11, 1160 – 1171


Keßen, S., Teutloff, C., Kern, J., Zouni, A., Bittl, R., High-Field 2H-Mims-ENDOR spectroscopy on PSII single crystals: Hydrogen Bonding of Y.D  ChemPhysChem 2010, 11, 1275-1282


Brose K, Tschirner N., Zouni A., Müh F., Yanagi K., Kataura H., Thomson C., Maultzsch J., Polarised Raman measurements of β-carotene encapsulated in SWNTs. Phys. Status Solid B 2010, 247, Nos. 11-12, 2871-2874




Kern, J., Zouni, A., Guskov, A., Krauss, N., Lipids in the Structure of Photosystem I, Photosystem II and the Cytochrome b6f Complex in H. Wada, N. Murata and Govindjee (eds.), Lipids in Photosynthesis: Essential and Regulatory Functions, PP. 203-241 Springer Science + Business Media B. V. 2009


Gabdulkhakov, A., Guskov, A., Broser, M., Kern, J., Müh, F., Saenger, W., Zouni, A., Probing the Accessibilty of the Mn4Ca Cluster in Photosystem II: Channels Calculation, Noble Gas Derivatization, and Cocrystallization with DMSO. Structure 17, 1223–1234, 2009


Teutloff, C., Pudollek, S., Kessen, S., Broser, M., Zouni, A., Bittl, R., Electronic structure of the tyrosine D radical and the water-splitting complex from pulsed ENDOR spectroscopy on photosystem II single crystals. Phys. Chem. Chem. Phys., 2009, 11, 6715-6726


Balaban, T.S., Braun, P., Hättig, C., Hellweg, A., Kern, J., Saenger, W., Zouni, A., Preferential pathways for light-trapping involving β-ligated chlorophylls. Biochim Biophys Acta, 1787 (2009), 1254-1265


Guskov,A., Kern,J., Gabdulkhakov,A, Broser,M., Zouni,A., Saenger,W., Cyanobacterial photosystem II at 2.9-Å resolution and the role of quinones, lipids, channels and chloride. Nat Struct Mol Biol 16, 334–342 (2009)




Loll, B., Broser, M., Kos, P. B., Kern, J., Biesiadka, J., Vass, I., Saenger, W., Zouni, A., Modeling of variant copies of subunit D1 in the structure of photosystem II from Thermosynechococcus elongatus. Biol. Chem., 389, 609-617, 2008


Müh, F., Renger, T, Zouni, A., Crystal structure of cyanobacterial photosystem II at 3.0 Å resolution: A closer look at the antenna system and the small membrane-intrinsic subunits. Plant Physiol. Biochem. 2008, 46, 238-264.


Müh, F., Zouni, A., Micelle formation in the presence of photosystem I. Biochim. Biophys. Acta 1778, 2008, 2298-2307


Zouni, A. (2008). From cell growth to the 3.0 Å resolution crystal structure of cyanobacterial photosystem II, in G. Renger (ed.) Primary processes of photosynthesis: Basic principles and apparatus, Vol. II, Royal Society Chemistry, Cambridge, pp 193-236


Yano, J., Kern, J., Pushkar, Y., Sauer, K., Glatzel, P., Bergmann, U., Messinger, J., Zouni, A., Yachandra, V., High-resolution structure of the photosynthetic Mn4Ca catalyst from X-ray spectroscopy. Philos. Trans. R. Soc. B 2008, 363, 1139-1147


Zein, S., Kulik, L.V., Yano, J., Kern, J., Pushkar, Y., Zouni, A., Yachandra, V.K., Lubitz, W., Neese, F., Messinger, J., Focusing the view on nature’s water-splitting catalyst. Phil. Trans. R. Soc. B 2008, 363, 1167-1177




Ishikita, H., Loll, B., Biesiadka, J., Kern, J., Irrgang, K.-D., Zouni, A., Saenger, W., Knapp, E.-W., Function of two β-carotenes near the D1 and D2 proteins in photosystem II dimers. Biochim. Biophys. Acta 1767, 2007, 79-87


Kern, J., Biesiadka, J., Loll, B., Saenger, W., Zouni, A., Structure of the Mn4Ca cluster as derived from X-ray diffraction. Photosynth Res. 92, 389-405 (2007)


Krivanek, R., Kern, J., Zouni, A., Dau, H., Haumann, M., Spare quinones in the QB cavity of crystallized photosystem II from Thermosynechococcus elongatus. Biochim. Biophys. Acta 1767, 2007, 520-527


Loll, B., Kern, J., W. Saenger, W., Zouni, A., Biesiadka, J., Lipids in Photosystem II: Interactions with protein and cofactors. Biochim. Biophys. Acta 1767, 2007, 509-519




Teutloff, C., Keßen, S., Kern, J., Zouni, A., Bittl, R., High-field (94-GHz) EPR spectroscopy on the S2 multiline signal of photosystem II. FEBS Lett. 580 (2006), 3605-3609


Yano, J, Kern, J., Sauer, K., Latimer, M. J., Pushkar, Y., Biesiadka, J., Loll, B., Saenger, W., Messinger, J., Zouni, A., Yahandra, V. K., Where water is oxidized to dioxygen: Structure of the photosynthetic Mn4Ca cluster. Science, 2006, 314, 821-825


Zimmermann, K, Heck, M., Frank, J., Kern, J., Vass, I., Zouni, A., Herbicide binding and thermal stability of photosystem II isolated from Thermosynechococcus elongatus. Biochim. Biophys. Acta 1757, 2006, 106-114




Kern, J., Loll, B., Lüneberg, C., DiFiore, D., Biesiadka, J., Irrgang, K-D., Zouni, A., Purification, characterisation and crystallisation of photosystem II from Thermosynechococcus elongatus cultivated in a new type of photobioreactor. Biochim. Biophys. Acta 1706, 2005, 147-157


Kern, J., Loll, B., Zouni, A., Saenger, W., Irrgang, K.-D., Biesiadka, J., Cyanobacterial photosystem II at 3.2 Å resolution - the plastoquinone binding pockets. Photosynth Res. 84, 153-159 (2005)


Loll, B., Kern, J., Saenger, W. Zouni, A., Biesiadka, J., Towards complete cofactor arrangement in the 3.0 Å resolution structure of photosystem II. Nature 438, 1040-1044 (2005)


Loll, B., Kern, J., Zouni, A., Saenger, W., Biesiadka, J., Irrgang, K.-D., The antenna system of Photosystem II from Thermosynechococcus elongatus at 3.2 Å resolution. Photosynth. Res. 86, 175-184 (2005)


Müh, F. Zouni, A., Extinction coefficients and critical solubilisation concentrations of photosystems I and II from Thermosynechococcus elongatus. Biochim. Biophys. Acta 1708, 2005, 219-228


Yano, J., Kern, J., Irrgang, K.-D., Latimer, M., Bergmann, U., Glatzel, P., Pushkar, Y., Biesiadka, J., Loll, B., Sauer K., Messinger, J., Zouni, A., Yachandra, V. K., X-ray damage to the Mn4Ca complex in single crystals of photosystem II: A case study for metalloprotein crystallography. Proc. Natl. Acad Sci. U. S. A. 2005, 102, 12047-12052


Zouni, A., Kern, J., Frank, J., Hellweg, T., Behlke, J., Saenger, W., Irrgang, K.-D., Size determination of cyanobacterial and higher plant photosystem II by gel permeation chromatography, light scattering and ultracentrifugation. Biochemistry 2005, 44, 4572-4581




Biesiadka, J., Loll, B., Kern, J., Irrgang, K-D., Zouni, A., Crystal structure of cyanobacterial photosystem II at 3.2 Å resolution: a closer look at the Mn-cluster. Phys. Chem. Chem. Phys., 2004, 6, 4733-4736


Kern, J., Biesiadka, J., Loll, B., Zouni, A., Irrgang, K.-D., Saenger, W. (2004). Crystal structure of cyanobacterial photosystem II at 3.2 Å resolution, in Photosynthesis: Fundamental Aspects to Global Perspectives, A. van der Est and D. Bruce, eds (Allen Press, Lawrence (KS), USA), pp. 230-234


Yano, J., Kern, J., Sauer, K., Messinger, J., Zouni, A., Yachandra, V. K. (2004). Mn4Ca Cluster of the water-oxidation enzyme studied by polarized X-ray spectroscopy of PS II single crystals. In Photosynthesis: Fundamental Aspects to Global Perspectives, A. van der Est and D. Bruce, eds (Allen Press, Lawrence (KS), USA), pp. 234-236


Zouni, A., Kern, J., Loll, B., Biesiadka, J., Saenger, W., Irrgang, K.-D. (2004). Biochemical and functional analysis of Photosystem II crystals from Thermosynechococcus elongatus diffracting to 3.2 Å, in Photosynthesis: Fundamental Aspects to Global Perspectives, A. van der Est and D. Bruce, eds (Allen Press, Lawrence (KS), USA), pp. 259-261




Sarrou, J., Isgandarova, S., Kern, J., Zouni, A., Renger, G., Lubitz, W., and Messinger, J., Nitric oxide-induced formation of the S-(2) state in the oxygen-evolving complex of photosystem II from Synechococcus elongatus. Biochemistry, 2003, 42 (4), 1016–1023




Fromme, P., Kern, J., Loll, B., Biesiadka, J., Saenger, W., Witt, H.T., Krauss, N., and Zouni, A., Functional implications on the mechanism of the function of photosystem II including water oxidation based on the structure of photosystem II. Philos. Trans. R. Soc. Lond. B 357: 1337-1345 (2002)




Hofbauer, W., Zouni, A., Bittl, R., Kern, J., Orth, P., Lendzian, F., Fromme, P., Witt, H.-T., and Lubitz, W., Photosystem II single crystals studied by EPR spectroscopy at 94 GHz: the tyrosine radical Y. Proc. Natl. Acad. Sci. U. S. A. 2001, 98, 6623-6628


Irrgang, K.-D., Georgalis, Y., Franke, P., Behlke, J., Saenger, W., and Zouni, A. (2001). Structural comparison of oxygen-evolving photosystem II core complexes from Synechococcus elongatus and Spinacia oleracea. In 12th International Conference on Photosynthesis (Brisbane, Australia: CSIRO Publishers, Melbourne, Australia), pp. S5-028


Vasil'ev, S., Orth, P., Zouni, A., Owens, T.G., and Bruce, D., Excited-state dynamics in photosystem II: insights from the x-ray crystal structure. Proc. Natl. Acad. Sci. U. S. A. 2001, 98, 8602-8607


Witt, H.T., Zouni, A., Kern, J., Fromme, P., Krauss, N., Saenger, W., and Orth, P. (2001). Crystal structure of Photosystem II and aspects of its function. In 12th International congress on photosynthesis (Brisbane: CSIRO Publishers, Melbourne, Australia), pp. PL01


Zouni, A., Witt, H.T., Kern, J., Fromme, P., Krauss, N., Saenger, W., and Orth, P., Crystal structure of photosystem II from Synechococcus elongatus at 3.8 A resolution. Nature 409, 739-743 (2001)


Zouni, A., Kern, J., Loll, B., Fromme, P., Witt, H.T., Orth, P., Krauss, N., Saenger, W., and Biesiadka, J. (2001). Biochemical characterization and crystal structure of water oxidizing photosystem II from Synechococcus elongatus. In 12th International congress on photosynthesis (Brisbane: CSIRO Publishers, Melbourne, Australia), pp. S05-003


vor 2001


Zouni, A., Lüneberg, C., Fromme, P., Schubert, W.D., Saenger, W., and Witt, H.T. (1998). Characterization of single crystals of Photosystem II from the thermophilic cyanobacterium Synechococcus elongatus. In Photosynthesis: Mechanisms and Effects, G. Garab, ed (Dordrecht: Kluwer Academic), pp. 925-928


Zouni, A., Jordan, R., Schlodder, E., Fromme, P., and Witt, H.T., First photosystem II crystals capable of water oxidation. Biochimica et Biophysica Acta 1457 (2000) 103–105